Pengaruh Metode Ekstraksi Maceration Vortex Technique terhadap Total Flavonoid Content dari Daun Meniran (Phyllanthus niruri L.) Effect of Maceration Vortex Technique Extraction Method on Total Flavonoid Content of Meniran Leaves (Phyllanthus niruri L.)
Main Article Content
Abstract
Phyllanthus niruri L., a medicinal plant from the Phyllanthaceae family, is widely recognized in traditional medicine for its flavonoid content in the leaves, which functions as a natural antioxidant protecting cells from damage caused by free radicals. This study aims to investigate the effect of the Maceration Vortex Technique (MVT) on the total flavonoid content of Phyllanthus niruri leaves. The method involved a 2-hour maceration process followed by 5 minutes of vortexing using 70% ethanol as the solvent. Total flavonoid content was analyzed using UV-Vis spectrophotometry at a maximum wavelength of 431 nm. The results demonstrated that the MVT method yielded a high flavonoid content, indicating the technique's effectiveness in extracting flavonoid compounds from Phyllanthus niruri leaves. The study concludes that MVT is a promising alternative extraction method that is efficient, simple, and applicable for obtaining bioactive compounds from natural sources. These findings have positive implications for the development of antioxidant-rich herbal products derived from natural ingredients.

Citation Metrics:
Downloads
Article Details

Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
References
Carmagnani, H. J., Mansano, G. B., & Sobreira, F. (2020). Optimization of the extraction process of Phyllanthus niruri L. Mundo da Saude, 44(1), 134–143. https://doi.org/10.15343/0104-7809.202044134143
Dewi, I. S., Saptawati, T., & Rachma, F. A. (2021). Skrining fitokimia ekstrak etanol kulit dan biji terong belanda (Solanum betaceum Cav.). Prosiding Seminar Nasional UNIMUS, 4, 1210–1218. https://prosiding.unimus.ac.id/index.php/semnas/article/view/894
Ghafar, F., Tengku Nazrin, T. N. N., Mohd Salleh, M. R., Nor Hadi, N., Ahmad, N., & Azahari, A. (2017). Total phenolic content and total flavonoid content in Moringa oleifera seed. Science Heritage Journal, 1(1), 23–35. https://doi.org/10.26480/gws.01.2017.23.35
Higea, J. F., Rivai, H., Septika, R., & Boestari, A. (2013). Karakterisasi ekstrak herba meniran (Phyllanthus niruri Linn) dengan analisa fluoresensi. Jurnal Ilmu Kefarmasian, 5(2). http://dx.doi.org/10.52689/higea.v5i2.84
Islam, M. T., Paz, M. F. C. J., Islam, B., De Alencar, M. V. O. B., & Melo-Cavalcante, A. A. D. C. (2016). Maceration-vortex-technique (MVT), a rapid and new extraction method in phyto-pharmacological screening. International Journal of Pharmacy and Pharmaceutical Sciences, 8(6), 1–3. https://innovareacademics.in/journals/index.php/ijpps/article/view/12045
Kardinan, A., & Kusuma, F. R. (2004). Meniran penambah daya tahan tubuh alami. Agromedia Pustaka. https://books.google.co.id/books?id=EeGpCgAAQBAJ
Kumar, A., Nirmal, P., Kumar, M., Jose, A., Tomer, V., Oz, E., Proestos, C., Zeng, M., Elobeid, T., Sneha, V., & Oz, F. (2023). Major phytochemicals: Recent advances in health benefits and extraction method. Molecules, 28(2), 1–41. https://doi.org/10.3390/molecules28020887
Luliana, S., Riza, H., & Indriyani, E. N. (2019). The effect of extraction method on total phenolic content and antioxidant activity of salam leaves (Syzygium polyanthum) using DPPH (1,1-diphenyl-2-picrylhidrazil). Majalah Obat Tradisional, 24(2), 72–76. https://doi.org/10.22146/mot.33955
Nguang, S. L., Yeong, Y. L., Pang, S. F., & Gimbun, J. (2017). Ultrasonic assisted extraction on phenolic and flavonoid content from Phyllanthus niruri plant. Indian Journal of Science and Technology, 10(2). https://doi.org/10.17485/ijst/2017/v10i2/110391
Parbuntari, H., Azra, F., Suryani, O., & Melati, M. (2023). Microencapsulation of flavonoids from gedi leaves (Abelmoschus manihot L.) in β-cyclodextrin using coprecipitation and kneading methods. Rasayan Journal of Chemistry, 16(2), 1035–1041. https://doi.org/10.31788/RJC.2023.1628287
Parbuntari, H., Etika, S. B., Mulia, M., & Delvia, E. (2019). A preliminary screening of the different of secondary metabolites ruku-ruku leaves (Ocimum tenuiflorum Linnen) in West Sumatera. Eksakta: Berkala Ilmiah Bidang MIPA, 20(2), 17–24. https://doi.org/10.24036/eksakta/vol20-iss2/193
Patel, J. R., Tripathi, P., Sharma, V., Chauhan, N. S., & Dixit, V. K. (2011). Phyllanthus amarus: Ethnomedicinal uses, phytochemistry and pharmacology: A review. Journal of Ethnopharmacology, 138(2), 286–313. https://doi.org/10.1016/j.jep.2011.09.040
Putri, C. N., Rahardhian, M. R. R., & Ramonah, D. (2022). Pengaruh metode ekstraksi terhadap kadar total fenol dan total flavonoid ekstrak etanol daun insulin (Smallanthus sonchifolius) serta aktivitas antibakteri terhadap Staphylococcus aureus. JPSCR: Journal of Pharmaceutical Science and Clinical Research, 7(1), 15. https://doi.org/10.20961/jpscr.v7i1.43465
Sabdoningrum, E. K., Hidanah, S., & Chusniati, S. (2021). Characterization and phytochemical screening of meniran (Phyllanthus niruri Linn) extract’s nanoparticles used ball mill method. Jurnal Ilmiah, 13(6), 1568–1572.10.5530/pj.2021.13.200
Soesanto, L. (2021). Dahsyatnya meniran hijau: Gempur berbagai penyakit dan sebagai anti-virus. https://books.google.co.id/books/about/DAHSYATNYA_MENIRAN_HIJAU.html?id=zCI6EAAAQBAJ&redir_esc=y
Tambunan, R. M., Swandiny, G. F., & Zaidan, S. (2019). Uji aktivitas antioksidan dari ekstrak etanol 70% herba meniran (Phyllanthus niruri L.) terstandar. Jurnal Ilmiah, 12(2), 60–64.https://doi.org/10.37277/sfj.v12i2.444
Tandi, J., Melinda, B., Purwantari, A., & Widodo, A. (2020). Analisis kualitatif dan kuantitatif metabolit sekunder ekstrak etanol buah okra (Abelmoschus esculentus L. Moench) dengan metode spektrofotometri UV-Vis. KOVALEN: Jurnal Riset Kimia, 6(1), 74–80. https://doi.org/10.22487/kovalen.2020.v6.i1.15044
Wang, Q., Jin, J., Dai, N., Han, N., Han, J., & Bao, B. (2016). Anti-inflammatory effects, nuclear magnetic resonance identification, and high-performance liquid chromatography isolation of the total flavonoids from Artemisia frigida. Journal of Food and Drug Analysis, 24(2), 385–391. https://doi.org/10.1016/j.jfda.2015.11.004














