Assessment of HIV 1 GP120/CD4 Binding Inhibition Potential of Methanolic Extracts of Achyranthes Aspera Leaves
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113-127
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10.58578/ajmsphr.v2i2.5184
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Authors:
Isaac John Umaru1, Tensaba Andes Akafa2, Ingwu Joseph Akem3, Ocheifa Mathew Ngbede4, Joseph Oteng5, Ashaka Fidelis Utioukpan6, Aboki Nwunuji Mijinyawa7, Odok Endurance Akam8, Ogholo Ogholo Ekup9 
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Abstract
Achyranthes aspera (Amaranthaceae) is an important medicinal herb found as a weed throughout Nigeria. Though almost all of its parts are used in traditional systems of medicines, leaves, seeds, roots and shoots are the most important parts which are used medicinally. The present article gives an account of updated information on its phytochemical and pharmacological properties. The Human immunodeficiency virus (HIV) infection is still contributing significantly in morbidities and mortalities in the world today. The drugs normally used to treat the infection are costly, toxic, and less effective due to resistance by HIV. Thus, the assessment of gp120-CD4 binding inhibition potential of Achyranthes aspera leaves extract was conducted using gp120-CD4 capture ELISA kits. Aqueous, methanol, and petroleum ether extracts were prepared at 1000, 500 and 250 μg/ml and tested for gp120-CD4 binding inhibition. Sub-cute toxicity assay was done using albino rats; Biochemical parameters including alanine amino transferase (ALT), aspartate amino transferase (AST) and alkaline phosphatase (ALP) as well as bilirubin for liver and urea, electrolytes and creatinine for kidney functions were evaluated. The results of the effect of crude aqueous, methanol and petroleum ether Achyranthes aspera leaves extract against gp120-CD4 showed inhibition ranging from 1.4 – 17.2 %. with 1000 μg/ml showing highest percentage of inhibition. There was no significant difference (P = 0.862) in terms of percentage inhibition between the three concentrations tested. Methanol extract demonstrated the highest percentage inhibition of gp120-CD4 bindings (17.2 %). No significant difference (P = 0.124) between the three extracts against gp-120-CD4 bindings was observed. The results of the sub-acute toxicity study have shown that, there were no physical changes in animals treated with 500 mg/kg of all the extracts. The result of liver function test revealed that, ALT, AST and ALP were within the normal range (12U/L) for both the high and low concentrations of the extracts including the control. Also result for total protein, albumin, globulin for the test albino rats and that of the control rat were found to be within the normal range 5.1-6.1, 4.2-5.3, and 3.1-3.8 g/dl respectively. For serum electrolyte level, sodium and potassium ions for the various concentrations of the extracts tested and control were also found to be within normal range. The result of kidney function test revealed that, urea, creatinine and direct and total bilirubin of the rats tested and control were all found to be within normal range. In conclusion, ether Achyranthes aspera leaves extract possessed some levels of HIV-1 gp120-CD4 binding inhibition potentials and the extracts was found to be non-toxic at 250 and 500mg/ kg body weight. It can be recommended that, the bioactive compounds should therefore be isolated and tested for gp120-CD4 binding inhibition activity.
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References
2. Rege A, Ambaye RY, Deshmukh RA. In-vitro testing of anti-HIV activity of some medicinal plants. Indian Journal Natural Product Resource. 2010;1(2):193–9.
3. Wyatt, G. E., Moe, A., & Guthrie, D. (1999). The gynaecological, reproductive, and sexual health of HIV-positive women. Cultural Diversity and Ethnic Minority Psychology, 5(3), 183.
4. Chan, D.C., and Kim, P.S. (1998). "HIV entry and its inhibition". Cell. 93 (5): 681–4.
5. Wyatt, G. E., Moe, A., & Guthrie, D. (1999). The gynaecological, reproductive, and sexual health of HIV-positive women. Cultural Diversity and Ethnic Minority Psychology, 5(3), 183.
6. Fletcher, C.V., Kakuda T.N., and Collier A.C. (2002). Bone and joints infections. Pharmacotherapy- a pathophysiologic approach. McGraw-Hill Medical Publishing Division, United States of America, 5th edition, pp. 2151–2174
7. Cos P., Arnold J.V., Dirk V.B. and El-Bala (2004). Anti-infective potential of natural product. Journal of Ethno pharmacology 106 (3): 290-302
8. Klos, M., Vandeventer, M., Milne, P.J., and Oosthuizen, V. (2009); in-vitro anti-HIV activity of five selected south African medicinal plant extracts. Journal of virology 124 (2): 182-188.
9. World Health Organization (2009): WHO’s role and responsibilities in health
10. Bako S.P., Bakfur I.J. and El-Bala (2005). Ethnomedicinal and phytochemical profile of some savanna plant species in Nigeria. International journal of botany. Volume 1 (2):147-150
11. Kayombo, E. J., Uiso F. C, Mbwambo Z. H, Mahunnah R.L, Moshi M. J, and Mgonda Y. H. (2007). Experience of initiating collaboration of traditional healers in managing HIV and AIDS in Tanzania. Journal of Ethnobiol. Ethnomed. 3:6.
12. Chinsembu, K.C. and Mutirua T (2008). Validation of traditional medicines for HIV/AIDs treatment in Namibia. A report of the study visits to Zambia and South Africa. Namibia, Windhoek, Namibia.
13. Jayakumar T, Sridhar MP, Bharathprasad TR, Ilayaraja MG, S Balasubramanian MP. Experimental studies of Achyranthes aspera (L) preventing nephrotoxicity induced by lead in albino rats. J Health Sci. 2009;55(5):701–8.
14. Khanna AK, Chander R, Singh C, Srivastava AK, Kapoor NK. Hypolipidemic activity of Achyranthes aspera L. in normal and triton induced hyperlipemic rats. Indian J Exp Biol. 1992;30(2):128–30.
15. Chakraborty A. Cancer chemo preventive activity of Achyranthes aspera leaves on Espstein bar virus activation and two stages mouse skin carcinogenesis. Cancer Lett. 2002;177(1):25–31.
16. Mishra SH, Bafna AR. Effect of methanol extract of Achyranthes aspera Linn. on rifampicin-induced hepatotoxicity in rats. Ars Pharm. 2004;45(4):343–51.
17. Shibeshi W, Makonnen E, Zerihun L, Debella A. Estrogenic activity in Ethanolic extract of Bupleram marginatum. J Pharmacol Afr Health Sci. 2006; 6(2):108–12.
18. Saravanan P, Ramasamy V, Shivakumar T. Antimicrobial activity of leaf extracts of Achyranthes aspera L. Asian J Chem. 2008;20(1):823–5.
19. Gayathri DS, Archanah A, Abiramasundari P, Priya V, Uma K, Abirami T. Pharmacological activities of Achyranthes aspera, an overview. Indian J Nutr Diet. 2009;46(12):485–90.
20. Barua CC, Talukdar A, Begum SA, Buragohain B, Roy JD, Borah RS, Lahkar M. Antidepressant like effects of Achyranthes aspera L. animals’ models of depression. Pharmacol. 2009; 2:587–94.
21. Paul DD, De KM, Ali K, Chatterjee DK, Nandi DG. Effects of various extracts from the roots of Achyranthes aspera. Contraception. 2010;81(4):355–61.
22. Khan MT, Ahmad K, Alvi MN, Mansoor B, Asif SM, Khan FZ, et al. Antibacterial and irritant activities of organic solvent extracts of Agave americana Linn., Albizia lebbeck Benth., Achyranthes aspera Linn. And Abutilon indicum Linn. - a preliminary investigation. Pakistan J Zool. 2010; 2(1):93–7.
23. Tariq, J., Usman A.A., Sana, R. and Sidra, R. (2011). In-vitro antiviral activity Solanum nigrum against Hepatitis C Virus. journal of virology; 8:26
24. Adetuyi, A.O., and Papoola, A.V. (2001). Phytochemical evaluation, Antimicrobial activity and determination of bioactive components from the leaves of Solanum nigrum. International journal of Biomedical research. Volume 2014. Pp11. http://dx.doi.org/10.1155/2014/4976
25. Salehi B., Nanjangud V and Bilge S. (2018). Medicinal plants used in the treatment of HIV. International Journal of Science. Doi:10.3390/ijms19051459 19(5):1459
26. Rege, A.A., Ramkrishna, Y.A. and Rayana, A.D. (2009). In-vitro testing of anti-HIV activity of some medicinal plants. Indian Journal of Natural Products and resources 1(2): 193 –199.
27. Reitman S, and Frankel S. (1957). A colorimetric method for the determination of serum glutamic oxaloacetic acid and glutamic pyruvate transaminases. American Journal of Clinical Pathology. 28:56–63.
28. Cheesbrough, M (2000): District laboratory practice in tropical countries. 3rd Edition, Tedford publishers: 167-171.Reitman et al. (2007);
29. Suryavathi, V., Sharma, S., Sharma, S., and Saxena, P., (2005). Acute toxicity of textile dye waste waters (untreated and treated) of sanganer on male reproductive systems of albino rats and mice toxicology 19(4): 547 -556.
30. Ryan, C. F., Bill, R.J. and Randall S. A. (2009): HIV typed entry inhibitors with a new mode of action. International Medical Press for Antiviral chemistry and chemotherapy. 19: 243-255.
31. Swanson, M.D., Winter H.C., Goldstein I.J., and Markovitz D.M. (2010). A lectin isolated from bananas is a potent inhibitor of HIV replication. Journal of Biochemistry. 285: 8646-8655.
32. Gustafson, K.R. (1992). The guttiferones, HIV-inhibitory benzophenones from Symphonia globulifera, Garcinia livingstonei, Garcinia ovalifolia and Clusia rosea. Tetrahedron Lett. 48: 10093–10102.
33. Lindequist, U., Niedermeyer T.H.J. and Julich W.D. (2005). The pharmacological potential of mushrooms: Evidence-based. Complim. Alternat. Med. 2(3): 285-299.Neurath et al., 2004
34. Calabrese, C. (2002). A phase I trial of andrographolide in HIV positiveYao et al., 2002 patients and normal volunteers. Phytother. Res. 14: 333–338.
35. Park, I.W., Han C, Song X, Green L.A., Wang T., Liu Y., Cen C., Song X., Yang B., Chen G, He J.J. (2009). Inhibition of HIV-1 entry by extracts derived from traditional Chinese medicinal herbal plants. BMC Compliment. Alternat. Med. 9:29. doi:10.1186/1472-6882-9-29.
36. Geuenich, S., Goffine C., Venzke S., Nolkemper S., Baumann I., Plinkert P., Reichling J. and Kepper O.T. (2008). Aqueous extracts from peppermint, sage and lemon balm leaves display potent anti-HIV activity by increasing the virion density. Retrovirology 5:27, doi:10.1186/1742-4690-5-27 http://www.retrovirology.com/cont ents/5/1/27
37. Häussinger, D. (2011, March). Hepatic encephalopathy. In Gut and Liver: Falk Symposium 174, Beijing, August 2010 (Vol. 174, p. 117). Karger Medical and Scientific Publishers.