The Anti-Sickling and Oxidative Properties of Ethanol Leaf Extract of Ocimum gratissimum L. (Scent Leaf)
Article Sidebar
Page Numbers:
155-170
Digital Object Identifier:
10.58578/ajcmpr.v3i2.9092
Viewed: 179 times
Downloaded: 94 times
LYAS Publisher cordially invites qualified professionals to serve as Editors or Reviewers. Your expertise will make an important contribution to maintaining and strengthening the academic quality of our publications. Interested applicants are kindly requested to complete the application form at the following link: Editors & Reviewers
Please feel free to contact us if you need any further information about the submission process or if you have any additional questions.
Authors:Onwubiko U. I.1, Imo C.2, Onwubiko G. N.3, Dawoye Y.4, Rayyanu S. S.5, Solomon J. E.6
Copyright :Authors retain copyright and grant the journal right of first publication.
Main Article Content
Abstract
This study evaluated the anti-sickling potential of the ethanol leaf extract of Ocimum gratissimum using microscopy-based assays. The extract markedly reversed 2% sodium metabisulfite–induced sickled red blood cells to normal biconcave forms, and microscopic observations indicated that the percentage reversal of sickled cells was both concentration- and time-dependent. At 250 μg/mL, the percentage reversal of sickled cells at 0, 20, and 30 minutes was 77.00%, 72.31%, and 71.77%, respectively, whereas the control (absence of extract) showed no reversal. At 500 μg/mL, the corresponding reversals were 76.19%, 69.60%, and 74.00%, while at 1000 μg/mL they were 71.49%, 75.92%, and 87.70%, respectively, demonstrating enhanced anti-sickling activity at higher extract concentrations over time. UV–Visible spectrophotometric analysis of oxyhaemoglobin revealed a gradual increase in the 630 nm band, consistent with the oxidation of ferrous (Fe²⁺) oxyhaemoglobin to ferric (Fe³⁺) methaemoglobin; the absorbance peak at 650 nm, attributable to chlorophyll, was clearly distinguishable from the haemoglobin oxidation band at 630 nm. In addition, the ethanol leaf extract of O. gratissimum exhibited a stabilizing effect on erythrocyte haemolysis. Phytochemical analysis showed the presence of aromatic and alkylic compounds, which may contribute to intracellular anti-sickling activity by interfering with haemoglobin S polymerization and, through their hydrophobic properties, may also modulate the erythrocyte lipid bilayer membrane.
Citation Metrics:
Citation Metrics & Similar Scopus Articles
-
Miryuk O.A. (2027)Technological and operational properties of composite magnesia bindersKompleksnoe Ispolzovanie Mineralnogo Syra, 342(3), 47-55
-
Mussin R.A. (2027)Prospects for Industrial Extraction of Methane from Coal Seams in the Karaganda Basin: Results of Experimental-Industrial Studies at the Taldykuduk SiteKompleksnoe Ispolzovanie Mineralnogo Syra, 342(3), 35-46
-
Fazeli F. (2027)The representation of the properties of the social actors in the two poetries of“Aghadar”and “Fokhos”written by Sheevan Foumani in accordance with the socio-semantic model and semio-semantic analysisLanguage Related Research, 17(4), 397-431
Article Details
References
Bhawnani, K., & Yadav, R. (2023). An overview on sickle cell anaemia. Journal of Pharmaceutical Negative Results, 14(S02), 2069–2080. https://www.pnrjournal.com/index.php/home/article/view/246
Cyril-Olutayo, M. C., Agbedahunsi, J. M., & Akinola, N. O. (2019). Studies on the effect of a nutritious vegetable, Telfairia occidentalis, on HbSS blood. Journal of Traditional and Complementary Medicine, 9(2), 156–162. https://doi.org/10.1016/j.jtcme.2017.08.013
Ekeke, G. I., & Shode, F. O. (1990). Phenylalanine is the predominant anti-sickling agent in Cajanus cajan seed extract. Planta Medica, 56(1), 41–43. https://doi.org/10.1055/s-2006-960880
Elekwa, I., Monanu, M. O., & Anosike, E. O. (2005). Effect of aqueous extracts of Zanthoxylum macrophyllum roots on membrane stability of human erythrocytes at different genotypes. Biokemistri, 17(1), 7–12. https://doi.org/10.4314/biokem.v17i1.32582
Faluyi, O. (2020). The medicinal values of scent leaf (Ocimum gratissimum). The Punch.
Fennell, C. W., Lindsey, K. L., McGaw, L. J., Stafford, G. I., Elgorashi, E. E., Grace, O. M., & van Staden, J. (2004). Assessing African medicinal plants for efficacy and safety: Pharmacological screening and toxicology. Journal of Ethnopharmacology, 94(2–3), 205–217. https://doi.org/10.1016/j.jep.2004.05.012
Franklyn, O. O., Paul, C. C., & Chinwendu, M. C. (2021). Sickle haemoglobin polymerization inhibition and anti-sickling of medicinal plants. Journal of Phytopharmacology, 10(2), 126–133. https://doi.org/10.31254/phyto.2021.10209
Ma, L., Yang, S., Peng, Q., Zhang, J., & Zhang, J. (2023). CRISPR/Cas9-based gene editing technology for sickle cell disease. Gene, 874, 147480. https://doi.org/10.1016/j.gene.2023.147480
Mehanna, A. S. (2001). Sickle cell anemia and anti-sickling agents then and now. Current Medicinal Chemistry, 8, 79–88. https://doi.org/10.2174/0929867013373778
Mukherjee, M., Rahaman, M., Ray, S. K., Shukla, P. C., Dolai, T. K., & Chakravorty, N. (2022). Revisiting fetal hemoglobin inducers in beta-hemoglobinopathies: A review of natural products, conventional and combinatorial therapies. Molecular Biology Reports, 49(3), 2359–2373. https://doi.org/10.1007/s11033-021-06977-8
Moody, J. O., Ojo, O. O., Omotade, O. O., Adeyemo, A. A., Olumese, P. E., & Ogundipe, O. O. (2003). Antisickling potential of a Nigerian herbal formula (ajawaron HF) and the major plant component (Cissus populnea L. CPK). Phytotherapy Research, 17, 1173–1176. https://doi.org/10.1002/ptr.1323