Effects of Cefuroxime and Varying Nitrogen Conditions on Growth and Pigment Composition of Chlorella sp.
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966-982
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10.58578/mikailalsys.v3i2.6116
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Authors:
Kalaboms Andrew Bitrus Bebwa1*, Daniel Biyenta Elijah2, Yerima Doka Dacip3 
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Abstract
This study addresses the limited research on the ecological impacts of cefuroxime on freshwater microalgae, despite the antibiotic’s widespread presence in aquatic environments. Specifically, it investigates the effects of cefuroxime on the growth and pigment composition, measured through cell density, chlorophyll-a, chlorophyll-b, total chlorophyll, and carotenoids of Chlorella sp. under nitrogen-replete and nitrogen-limited conditions. A quantitative experimental design was applied, comprising five treatment groups cultured in triplicate. Data collection involved microscopic cell counts and spectrophotometric analysis, while growth and pigment dynamics were assessed using specific growth rate equations and pigment concentration formulas. Statistical analyses included the Shapiro-Wilk test, Levene’s test, repeated measures ANOVA, and Duncan’s Multiple Range Test (p ≤ 0.05). Results indicated that cefuroxime initially stimulated growth and pigment production under nitrogen-replete conditions but led to significant inhibition over time. Conversely, the control group demonstrated the most stable growth and pigment accumulation by day seven. These outcomes challenge the notion that low-dose antibiotics are ecologically benign and underscore the critical role of nitrogen availability in modulating antibiotic effects. The study concludes that cefuroxime exerts time- and nutrient-dependent impacts on Chlorella sp., advancing ecotoxicological theory by elucidating the interactive effects of nutrient stress and pharmaceutical contamination. Practical implications highlight the necessity for stricter regulation of pharmaceutical effluents and improved nutrient management in aquatic systems. Future investigations are recommended to explore the combined effects of multiple antibiotics under varied nutrient regimes.
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References
Amirian, V., Kosari-Nasab, M. & Movafeghi, A. (2025). The capacity of the green microalga Chlorella vulgaris in overcoming the detrimental effects of cephalexin contamination. World Journal of Microbiol Biotechnology 41, 109. https://doi.org/10.1007/s11274-025-04329-3
Ansari, F. A., Hassan, H., Gani, K. M., Rawat, I., Gupta, S. K., & Bux, F. (2025). Influence of nitrogen sources on growth and biochemical composition of Chlorella sorokiniana. Biomass and Bioenergy, 197, 107832.
Benavente-Valdés, J. R., Aguilar, C., Contreras-Esquivel, J. C., Méndez-Zavala, A., & Montañez, J. (2016). Strategies to enhance the production of photosynthetic pigments and lipids in chlorophycae species. Biotechnology Reports, 10, 117-125.
Chia, M. A., Lombardi, A. T., Melão, M. D. G. G. and Parrish, C. C. (2013). Effects of cadmium and nitrogen on lipid composition of Chlorella vulgaris (Trebouxiophyceae, Chlorophyta). European Journal of Phycology, 48(1), 1–11. https://doi.org/10.1080/09670262.2012.750687
Circuncisão, A.R., Catarino, F.M., Gélinas, Y. and Hussner, A., (2022). Carbon and Nutrient Removal by Aquatic Plants and Algae: Potential for Wastewater Treatment, Biomass Valorization, and Environmental Sustainability. Sustainability, 14(3), p.1237
Das, N., Madhavan, J., Selvi, A., & Das, D. (2019). An overview of cephalosporin antibiotics as emerging contaminants: a serious environmental concern. 3 Biotech, 9(6), 231. https://doi.org/10.1007/s13205-019-1766-9.
Ewa Felis, Joanna Kalka, Adam Sochacki, Katarzyna Kowalska, Sylwia Bajkacz, Monika Harnisz, Ewa Korzeniewska, (2020). Antimicrobial pharmaceuticals in the aquatic environment - occurrence and environmental implications, European Journal of Pharmacology, Volume 866, 172813, ISSN 0014-2999, https://doi.org/10.1016/j.ejphar.2019.172813. (https://www.sciencedirect.com/science/article/pii/S0014299919307654)
Graham, L.E., Kodner, R.B., Fisher, M.M., Graham, J.M., Wilcox, L.W., Hackney, J.M., Obst, M., Bilkey, P.C., Hanson, D.T., Stiller, J.W. and Graham, J.E., (2021). Algae as primary producers, oxygenic phototrophs, and oxygenic symbionts: the evolutionary beginnings, early diversification, and subsequent onslaught of global warming, eutrophication, and ocean acidification. Journal of Phycology, 57(3), pp.950-994.
Guo, R., Xie, W., & Chen, J. (2015). Assessing the combined effects from two kinds of cephalosporins on green alga (Chlorella pyrenoidosa) based on response surface methodology.. Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, 78, 116-21 . https://doi.org/10.1016/j.fct.2015.02.007.
Guo, J., Peng, J., Lei, Y., Kanerva, M., Li, Q., Song, J., Guo, J., & Sun, H. (2019). Comparison of oxidative stress induced by clarithromycin in two freshwater microalgae Raphidocelis subcapitata and Chlorella vulgaris.. Aquatic toxicology, 219, 105376 . https://doi.org/10.1016/j.aquatox.2019.105376.
Hillebrand, H., Dürselen, C. D., Kirschtel, D., Pollingher, U., & Zohary, T. (1999). Biovolume calculation for pelagic and benthic microalgae. Journal of phycology, 35(2), 403-424.
Huang, F. L., Qin, L. T., Mo, L. Y., Zeng, H. H., & Liang, Y. P. (2024). Mechanism of the Synergistic Toxicity of Ampicillin and Cefazoline on Selenastrum capricornutum. Toxics, 12(3), 217.
Hutárová, L., Hlebová, M., Vešelenyiová, D., Krajčoviechová, I., & Strunecký, O. (2023). EFFECT OF SELECTED ANTIBIOTICS ON THE GROWTH AND MORPHOLOGY OF CYANOBACTERIA. Journal of microbiology, biotechnology and food sciences, 12(6), e10221-e10221.
Isabel T. Carvalho, Lúcia Santos, (2016). Antibiotics in the aquatic environments: A review of the European scenario, Environment International, Volume 94, Pages 736-757, ISSN 0160-4120, https://doi.org/10.1016/j.envint.2016.06.025. (https://www.sciencedirect.com/science/article/pii/S0160412016302434)
Jiang, M., Wang, L., & Ji, R. (2010). Biotic and abiotic degradation of four cephalosporin antibiotics in a lake surface water and sediment. Chemosphere, 80 11, 1399-405 . https://doi.org/10.1016/j.chemosphere.2010.05.048.
Kayode-Afolayan, S. D., Ahuekwe, E. F., & Nwinyi, O. C. (2022). Impacts of pharmaceutical effluents on aquatic ecosystems. Scientific African, 17, e01288. Chalew, J.C. and Halden C.T.I. 2009. “Effects tetracycline Treatment Containing Poultry Manure on Crude Oil Degradation in a Sandy Loan Soil”. Appl. Ecological. Environmental. Restoration. 3(1): 47-53.
Lemley, D. A., Adams, J. B., & Bate, G. C. (2016). A review of microalgae as indicators in South African estuaries. South African Journal of Botany, 107, 12-20.
Lenart-Boroń, Anna & Prajsnar, Justyna & Guzik, Maciej & Boroń, Piotr & Chmiel, Maria. (2020). How much of antibiotics can enter surface water with treated wastewater and how it affects the resistance of waterborne bacteria: A case study of the Białka river sewage treatment plant. Environmental Research. 191. 110037. 10.1016/j.envres.2020.110037.
Li, X., Li, W., Zhai, J., Wei, H., & Wang, Q. (2019). Effect of ammonium nitrogen on microalgal growth, biochemical composition and photosynthetic performance in mixotrophic cultivation. Bioresource technology, 273, 368-376.
Li, Z., Gao, X., Bao, J., Li, S., Wang, X., Li, Z., & Zhu, L. (2022). Evaluation of growth and antioxidant responses of freshwater microalgae Chlorella sorokiniana and Scenedesmus dimorphus under exposure of moxifloxacin.. The Science of the total environment, 159788 . https://doi.org/10.1016/j.scitotenv.2022.159788.
Miguel González-Pleiter, Soledad Gonzalo, Ismael Rodea-Palomares, Francisco Leganés, Roberto Rosal, Karina Boltes, Eduardo Marco, Francisca Fernández-Piñas, (2013). Toxicity of five antibiotics and their mixtures towards photosynthetic aquatic organisms: Implications for environmental risk assessment, Water Research, Volume 47, Issue 6, Pages 2050-2064, ISSN 0043-1354, https://doi.org/10.1016/j.watres.2013.01.020. (https://www.sciencedirect.com/science/article/pii/S0043135413000377)
Negi, S., Barry, A. N., Friedland, N., Sudasinghe, N., Subramanian, S., Pieris, S., ... & Sayre, R. (2016). Impact of nitrogen limitation on biomass, photosynthesis, and lipid accumulation in Chlorella sorokiniana. Journal of applied phycology, 28, 803-812.
OECD (2011), Development Co-operation Report 2011: 50th Anniversary Edition, OECD Publishing, Paris, https://doi.org/10.1787/dcr-2011-en.
Pauletto, M., & De Liguoro, M. (2024). A review on fluoroquinolones’ toxicity to freshwater organisms and a risk assessment. Journal of Xenobiotics, 14(2), 717-752.
Prateek Sharma, Lata Rani, Ajmer S. Grewal, Arun Lal Srivastav, (2022). Chapter2 - Impact of pharmaceuticals and antibiotics waste on the river ecosystem: a growing threat, Editor(s): Sughosh Madhav, Shyam Kanhaiya, Arun Srivastav, Virendra Singh, Pardeep Singh, Ecological Significance of River Ecosystems, Elsevier, Pages 15-36, ISBN 9780323850452, https://doi.org/10.1016/B978-0-323-85045-2.00015-7. (https://www.sciencedirect.com/science/article/pii/B9780323850452000157)
Ribeiro, A. R. (2017). Fate and effects of two veterinarian cephalosporins, ceftiofur and cefapirin, in the aquatic environment (Doctoral dissertation, Duisburg, Essen, Universität Duisburg-Essen).
Ribeiro, Alyson & Sures, Bernd & Schmidt, Torsten. (2018). Cephalosporin antibiotics in the aquatic environment: A critical review of occurrence, fate, ecotoxicity and removal technologies. Environmental Pollution. 241. 10.1016/j.envpol.2018.06.040.
Ritchie, R. J. (2006). Consistent sets of spectrophotometric chlorophyll equations for acetone, methanol and ethanol solvents. Photosynthesis. Restoration, 89, 27–41.
Rydzyński, D., Piotrowicz-Cieślak, A. I., Grajek, H., & Wasilewski, J. (2019). Investigation of chlorophyll degradation by tetracycline. Chemosphere, 229, 409-417.
Tarazona Delgado, R., Guarieiro, M. D. S., Antunes, P. W., Cassini, S. T., Terreros, H. M., & Fernandes, V. D. O. (2021). Effect of nitrogen limitation on growth, biochemical composition, and cell ultrastructure of the microalga Picocystis salinarum. Journal of Applied Phycology, 33, 2083-2092.
Truong, T., Park, Y., Winarto, J., Huynh, P., Moon, J., Choi, Y., Song, D., Koo, S., & Kim, S. (2024). Understanding the Impact of Nitrogen Availability: A Limiting Factor for Enhancing Fucoxanthin Productivity in Microalgae Cultivation. Marine Drugs, 22. https://doi.org/10.3390/md22020093.
Ugya, A. Y., Chen, H., & Wang, Q. (2024). Microalgae biofilm system as an efficient tool for wastewater remediation and potential bioresources for pharmaceutical product production: an overview. International Journal of Phytoremediation, 26(1), 131-142.
Wang, M., Zhang, Y., & Guo, P. (2018). "Effect of florfenicol and thiamphenicol exposure on the photosynthesis and antioxidant system of Microcystis flos-aquae." Aquatic Toxicology, 186, 67–76. https://doi.org/10.1016/ journal of aquatic toxicology.2018.02.022
Wang, X., Dou, X., Wu, J., & Meng, F. (2021). Attenuation pathways of erythromycin and biochemical responses related to algal growth and lipid synthesis in a microalgae-effluent system.. Environmental research, 110873 . https://doi.org/10.1016/j.envres.2021.110873.
Wang, C., Zheng, Y., Li, R., Yin, Q., & Song, C. (2022). Removal of cefradine by Chlorella sp. L166 and Scenedesmus quadricauda: Toxicity investigation, degradation mechanism and metabolic pathways. Process Safety and Environmental Protection. https://doi.org/10.1016/j.psep.2022.02.064.
Wu, N., Dong, X., Liu, Y., Wang, C., Baattrup-Pedersen, A., & Riis, T. (2017). Using river microalgae as indicators for freshwater biomonitoring: Review of published research and future directions. Ecological Indicators, 81, 124-131.
Xu. R, Z.H. Yang, Y. Zheng, Q.P. Wang, Y. Bai, J.B. Liu, Y.R. Zhang, W.P. Xiong, Y. Lu and C.Z. Fan. (2019). Metagenomic analysis reveals the effects of long-term antibiotic pressure on sludge anaerobic digestion and antimicrobial resistance risk, Bioresources Technology. 282 179–188, https://doi.org/10.1016/j.biortech.2019.02.120.
Yaakob, M. A., Mohamed, R. M. S. R., Al-Gheethi, A., Aswathnarayana Gokare, R., & Ambati, R. R. (2021). Influence of Nitrogen and Phosphorus on Microalgal Growth, Biomass, Lipid, and Fatty Acid Production: An Overview. Cells, 10(2), 393. https://doi.org/10.3390/cells10020393
Yıldırım, A. (2025). Effect of the streptomycin removal process on metabolite profile of Chlamydomonas reinhardtii: the potential of antibiotic stress on lipid production for biodiesel. Journal of Applied Phycology, 37(1), 193-204.
Yu, Y., Zhou, Y., Wang, Z., Torres, O., Guo, R., & Chen, J. (2017). Investigation of the removal mechanism of antibiotic ceftazidime by green algae and subsequent microbic impact assessment. Scientific Reports, 7. https://doi.org/10.1038/s41598-017-04128-3.
Yu, C., Pang, H., Wang, J. H., Chi, Z. Y., Zhang, Q., Kong, F. T., ... & Che, J. (2022). Occurrence of antibiotics in waters, removal by microalgae-based systems, and their toxicological effects: A review. Science of The Total Environment, 813, 151891.
Zhao, Y., Huang, Y., Hu, S., Xu, T., Fang, Y., Liu, H., ... & Qu, R. (2023). Combined effects of fluoroquinolone antibiotics and organophosphate flame retardants on Microcystis aeruginosa. Environmental Science and Pollution Research, 30(18), 53050-53062.
Zhong, X., Zhu, Y., Wang, Y., Zhao, Q., & Huang, H. (2021). Effects of three antibiotics on growth and antioxidant response of Chlorella pyrenoidosa and Anabaena cylindrica. Ecotoxicology and Environmental Safety, 211, 111954.