Isolation and Molecular Characterisation of Chlorogonium sp. from Industrial Wastewater
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442-451
Digital Object Identifier:
10.58578/mikailalsys.v2i3.3851
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Authors:Ajiduku Leyoa Abershi1*, Joseph Ikwebe2, Ezeonu Chukwuma Stephen3, Alice Njolke Mafe4, Moses Adondua Abah5
Copyright :Authors retain copyright and grant the journal right of first publication.
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Abstract
Microalgae are photosynthetic, unicellular microorganisms also known as phytoplankton. They are small plant-like entities. In this research, the sample were collected from cement factory in a sterilised 20L container wrapped with foil paper and were transported down to Federal University, Wukari where it was kept in refrigerator at biochemistry laboratory. Blue-Green media (BG-11) was prepared. Wastewater containing Microalgae obtained from cement wastewater pond were cultivated in BG-11 medium to determine the growth of the organism. BG-11 medium contained macronutrients, trace metals and some vitamins which aid the growth of the organism. The wastewater sample containing with suspected microalgae was inoculated (inoculum ratio = 25%) and incubated under atmospheric CO2 at room temperature (30±2°C) in our laboratory for two to three weeks during the incubation period. In order to purify the isolates, the upper growth layer was first decanted into a freshly produced medium and then plated on BG-11 media that had been solidified with 1% agar-agar. For several of the cultures, growth on the agar plates continued for around three weeks. Following repeated sub-culturing, the emerging greenish colonies were re-emerged into a sterile BG-11 agar medium. In isolation of organism from the industrial cement waste water, the isolate was identified by morphological and molecular identification by extracting the DNA, run the electrophoretic analysis and PCR using primers for 18S rRNA eukaryotic microalgal and then run the sequence analysis. The results of this study obtained, indicated that, the electrophoretic result show the band has 1800-2000base pair and the organism isolated from the industrial cement waste water were chlorogonium sp. with a percent similarity of 78.65% and accession number of OR886595 based on data Gene Bank blast results.
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References
Ajiduku, L.A., Ikwebe, J. and Ezeonu, S.C. (2023), Isolation and molecular characterisation of Chlorogonium sp. from waste water and Its potentials application bioremediation and biodiesel production
Anderson R. (2011). Algae Culture Techniques. Elsevier Academic Press
Aullon AA. (2010). Biodiesel from Microalgae. J. Chem. Eng. and Technol.1-92.
Arceivala, S.J., Asolekar, S.R. (2006). Wastewater treatment for pollution control and reuse. New Delhi: Tata McGraw-Hill Publishing.
Arora, N., Patel, A., Sartaj, K., Pruthi, P. A., & Pruthi, V. (2016). Bioremediation of domestic and industrial wastewaters integrated with enhanced biodiesel production using novel oleaginous microalgae. Environmental Science and Pollution Research, 23(20), 20997-21007.
Ballesteros, I., Terán, P., Guamán-Burneo, C., González, N., Cruz, A., & Castillejo, P. (2021). DNA barcoding approach to characterize microalgae isolated from freshwater systems in Ecuador. Neotropical Biodiversity, 7(1), 170-183.
Chisti Y (2007). Biodiesel from microalgae. Biotechnol. Adv. 25(3):294-306.
Gerardo, M. L., Van Den Hende, S., Vervaeren, H., Coward, T., & Skill, S. C. (2015). Harvesting of microalgae within a biorefinery approach: A review of the developments and case studies from pilot-plants. Algal Research, 11, 248-262.
Dahiya, A. (2015). Algae biomass cultivation for advanced biofuel production. In Bioenergy (pp. 219-238). Academic Press.
Graham , L.E and Wilcox , L.(2000). In: Rye T., Tarabojkia, L. (eds.), Algae. First Edition. Prentice –Hill , Inc., United States of America.
Ifeanyi V.O., Ukogo Ifeoma and Ahaiwe, R.I. (2016). Isolation and identification of microalgae from fresh water sources. Proceedings of the 3rd NSM south East zonal symposium.
John D.M., Whitton B.A., Brook A.J. (2002). The Freshwater Algal Flora of the British Isles.Cambridge: Cambridge University Press. 1- 118.
Khaw, Y. S., Khong, N. M. H., Shaharuddin, N. A., & Yusoff, F. M. (2020). A simple 18S rDNA approach for the identification of cultured eukaryotic microalgae with an emphasis on primers. Journal of microbiological methods, 172, 105890.
Mooij, P. R., Stouten, G. R., Tamis, J., Van Loosdrecht, M. C., & Kleerebezem, R. (2013). Survival of the fattest. Energy & Environmental Science, 6(12), 3404-3406.
Milledge, J. J. (2011). Commercial application of microalgae other than as biofuels: a brief review. Reviews in Environmental Science and Bio/Technology, 10(1), 31-41.
Maity, J. P., Bundschuh, J., Chen, C. Y., & Bhattacharya, P. (2014). Microalgae for third generation biofuel production, mitigation of greenhouse gas emissions and wastewater treatment: Present and future perspectives–A mini review. Energy, 78, 104-113.
Ogbonna, I. O., Okpozu, O. O., Ikwebe, J., & Ogbonna, J. C. (2018). Utilisation of Desmodesmus subspicatus LC172266 for simultaneous remediation of cassava wastewater and accumulation of lipids for biodiesel production. Biofuels.
Okpozu, O. O., Ogbonna, I. O., Ikwebe, J., & Ogbonna, J. C. (2019). Phycoremediation of cassava wastewater by Desmodesmus armatus and the concomitant accumulation of lipids for biodiesel production. Bioresource technology reports, 7, 100255.
Popp J, Lakner Z, Harangi-Rákos M, Fári M. (2014). The effect of bioenergy expansion: food, energy, and environment. Renew Sust Energ Rev.32:559–78.
Spolaore, P., Joannis-Cassan, C., Duran, E., & Isambert, A. (2006). Commercial applications of microalgae. Journal of bioscience and bioengineering, 101(2), 87-96.
Stoermer, E.F., Kreis, R.G and Andresen , N . A. (1999). Checklist of Diatoms from the Laurentian
Great lakes. (1997). Journal of Great lakes Resources. 25(3): 515-566.
Shankar P.H. (2013). Fresh Water Algae as Indicators of Water Quality. Univ. J. Env Res. Technol. 3: 473-482.
Prasad, H., Lohchab, R.K., Singh, B., Nain, A. & Kumari, M. (2019) Lime treatment of wastewater in a plywood industry to achieve the zero liquid discharge. Journal of Cleaner Production, 240, 118176.
Prescott G. (2011). How to Know the Freshwater Algae. Pictured Key Series. Dubuque Iowa: prism. SDPI Res News Bulletin. 2007;14.
Priyadarshani, I., & Rath, B. (2012). Commercial and industrial applications of micro algae–A review. Journal of Algal Biomass Utilization, 3(4), 89-100.
Worker s, H., Barbosa , M., Kleirfgris, D . M. M, Bosama, R. and Wijiftals. (2011). Microalgae : The green gold of the future ? www.algae.wur.ni
Wang, Y., Ho, S. H., Cheng, C. L., Guo, W. Q., Nagarajan, D., Ren, N. Q., ... & Chang, J. S. (2016). Perspectives on the feasibility of using microalgae for industrial wastewater treatment. Bioresource Technology, 222, 485-497.
Zainul, R. (2016). Isolation and molecular identification of freshwater microalgae in Maninjau Lake West Sumatra. Der Pharmacia Lettre, 8(20), 177-187.