Analysis of Bioactive Constituents (HPLC) of Chloroform Leaf Extract from Kalanchoe pinnata in Takum, Taraba State Nigeria
Article Sidebar
Page Numbers:
332-339
Digital Object Identifier:
10.58578/ajstm.v1i1.3676
Viewed: 697 times
Downloaded: 196 times
LYAS Publisher cordially invites qualified professionals to serve as Editors or Reviewers. Your expertise will make an important contribution to maintaining and strengthening the academic quality of our publications. Interested applicants are kindly requested to complete the application form at the following link: Editors & Reviewers
Please feel free to contact us if you need any further information about the submission process or if you have any additional questions.
Authors:
Emmanuel Askebnde Joel1, Ubur Terzulum Wilson2, Isaac John Umaru3, Kingsley Iyoko Iseko4, Dafup Katdel Istifanus5, Gloria Omonefe Oladele6 
Copyright :
Authors retain copyright and grant the journal right of first publication.
Main Article Content
Abstract
HPLC techniques were employed to analyze the bioactive components of the chloroform leaf extract of Kalanchoe pinnata leaf from Takum in Taraba State. A botanist from Taraba State University's Department of Biological Sciences in Jalingo identified the plant leaves, which were then gathered, cleaned, and processed. Using cold maceration, extraction was done by weighing 1500g of the powdered leaf into 1 liter of ethanol and distill water, respectively. Until further investigation, the extracts were stored in a refrigerator in a firmly closed container. The injection valve was used for the HPLC analysis, and the conditions were 20 μl, a UV variable wavelength detector (set at 300 nm), reprosol 100 C8mn, 5 μm 4.6 x 150mm column (30°C), and sensitivity of 0.001. Phosphate buffer (v/v) was used as an aqueous solvent (A) and CH3CN as an organic solvent (B) in HPLC. Gradient elution of the analytes occurred at a flow rate of 1 milliliter per minute. Software generated chromatograms. Operating temperature of the HPLC instrument was room temperature (23 ± 2°C). After injecting 20 μl of each diluted extract into the HPLC three times, the average peak areas were produced and utilized for quantification. Based on the HPLC analysis, the chloroform leaf extract of Kalanchoe pinnata contained the following bioactive constituents: quercetin, gallic acid, and chlorogenic acid. Additionally, the presence of vitamins B1 and B3 was also noted. The different bioactive constituents eluted at different retention times, and their respective amounts were also detected.
Downloads
Article Details
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
References
Bhowmik D, Kumar KPS, Yadav A, Srivastava S, Paswan S, Dutta AS. Kalanchoe pinnata (Lam.) Pers. As a potent ethnopharmacological plant: A review. Journal of ethnopharmacology. 2020 Feb 10;250:112477.
Boots AW, Haenen GR, Bast A. Health effects of quercetin: from antioxidant to nutraceutical. Eur J Pharmacol. 2008;585(2-3):325-337.
Handa, S. S., Khanuja, S. P. S., Longo, G., & Rakesh, D. D. (2008). Extraction technologies for medicinal and aromatic plants (United Nations Industrial Development Organisation and the International Centre for Science and High Technology). International Centre for Science and High Technology-United Nations Industrial Development Organization, area Science Park Padriciano, 99, 34012.
Mitra P, Dey A, Chandra J. Phytochemical analysis, anti-oxidant and antibacterial activity of Kalanchoe pinnata leaf extract. Pharmacognosy research. 2018;10(4):386.
Nalini, R and Anuradha, R. (2017). Phytochemical Screening and Fluorescence Analysis of Methanolic Flower Extracts of Punica granatum L. Scholars Academic Journal of Pharmacy, 6(9), 378-82.
Nguyen VT, Nguyen MT, Nguyen NT, Nguyen LV, Pham HV, Nguyen TM. A review on the phytochemistry, medicinal properties, and potential uses of Kalanchoe pinnata (Lam.) Pers. Phytotherapy Research. 2019 Mar;33(3):547-67.
Ong KW, Hsu A, Tan BKH. Chlorogenic acid stimulates glucose transport in skeletal muscle via AMPK activation: a contributor to the beneficial effects of coffee on diabetes. PLoS One. 2018;13(9):e0198108.
Salehi B, Venditti A, Sharifi-Rad M, et al. The therapeutic potential of gallic acid and its derivatives: a patent review. Expert Opin Ther Pat. 2019;29(1):15-32.
Thenmozhi, M., & Rajeshwari, S. (2010). Phytochemical analysis and antimicrobial activity of Polyalthia longifolia. Int J Pharm Bio Sci, 1(3), 6288-99.