Effect of Bacillus megaterium on the Physio-Chemical and Compaction Characteristics of Silty Sand
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1816-1828
Digital Object Identifier:
10.58578/ajstea.v3i5.7478
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Authors:
Onah O. Mary1*, A. O. Eberemu2, K. J. Osinubi3 
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Abstract
Silty soil obtained from Wudil Local Government Area, Kano State—classified as A-3(0) under the AASHTO system and SP-SM under the Unified Soil Classification System (USCS)—was treated using the Microbial-Induced Calcite Precipitation (MICP) technique to enhance its geotechnical properties. The study investigated the effects of varying concentrations of Bacillus megaterium (0, 1.5 × 10⁸, 6.0 × 10⁸, 1.2 × 10⁹, 1.8 × 10⁹, and 2.4 × 10⁹ cells/ml) on the compaction and index properties of the soil. A premixing method was employed, and treated samples were prepared using bacterial suspension-to-cementation reagent ratios of 25:75, 50:50, and 75:25, with the control sample comprising 100% cementation solution. Results showed that the maximum dry density (MDD) was achieved at a bacterial concentration of 6.0 × 10⁸ cells/ml, corresponding with an optimum moisture content (OMC), indicating improved soil densification. The findings suggest that MICP treatment, particularly at optimal bacterial concentrations and reagent ratios, can enhance the compaction characteristics of silty sand, with implications for sustainable ground improvement techniques in civil engineering applications.
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References
Achal, V., Mukherjee, A., Basu, P. C., & Reddy, M. S. (2009). Strain improvement of Sporosarcina pasteurii for enhanced urease and calcite production. Journal of Industrial Microbiology and Biotechnology, 36(3), 433–438.
Achal, V., Mukherjee, A., & Reddy, M. S. (2013). Biogenic treatment improves the durability and remediates the cracks of concrete structures. Construction and Building Materials, 48, 1–5.
Alvarado, D. (2009). Bio-mediated Soil Improvement: Cementation of Unsaturated Sand Samples (PhD thesis). Arizona State University.
Anbu, P., Kang, C.-H., Shin, Y.-J., & So, J.-S. (2016). Formations of calcium carbonate minerals by bacteria and its multiple applications. SpringerPlus, 5(1), 250.
AASHTO. (1986). Standard Specifications for Transportation Materials and Methods of Sampling and Testing (14th ed.). Washington, D.C.: American Association of State Highway and Transportation Officials.
British Standards Institution. (1990). BS 1377: Methods of Testing Soils for Civil Engineering Purposes. London: BSI.
British Standards Institution. (1990). BS 1924: Methods of Test for Stabilized Soils. London: BSI.
Bu, C., Wen, K., Liu, S., Ogbonnaya, U., & Li, L. (2018). Development of bio-cemented constructional materials through microbial induced calcite precipitation. Materials and Structures, 51(1), 1–11.
Choi, S., Wu, S., & Chu, J. (2016). Biocementation of sand using eggshell as calcium source. Journal of Geotechnical and Geoenvironmental Engineering, 142(10).
Chu, J., Ivanov, V., Naeimi, M., Stabnikov, V., & Liu, H. L. (2014). Optimization of calcium-based bioclogging and biocementation of sand. Acta Geotechnica, 9, 277–285. https://doi.org/10.1007/s11440-013-0278-8
DeJong, J. T., Fritzges, M. B., & Nüsslein, K. (2006). Microbially induced cementation to control sand response to undrained shear. Journal of Geotechnical and Geoenvironmental Engineering, 132(11), 1381–1392.
DeJong, J. T., et al. (2013). Biogeochemical processes and geotechnical applications: Progress, opportunities and challenges. Géotechnique, 63(4), 287–301.
De Muynck, W., Verbeken, K., De Belie, N., & Verstraete, W. (2010). Influence of urea and calcium dosage on the effectiveness of bacterially induced carbonate precipitation on limestone. Ecological Engineering, 36(2), 99–111.
Dhami, N. K., Mukherjee, A., & Watkin, E. L. J. (2018). Microbial diversity and mineralogical-mechanical properties of calcitic cave speleothems under natural and in vitro conditions. Frontiers in Microbiology, 9, 40. https://doi.org/10.3389/fmicb.2018.00040
Eberemu, A. O., Bassey, A. O., & Osinubi, K. J. (2021). Evaluation of the free swell and physio-chemical properties of black cotton soil treated with Bacillus coagulans. IOP Conference Series: Materials Science and Engineering, 1036, 012030.
Ferris, F. G., & Stehmeier, L. G. (1992). Bacteriogenic mineral plugging. Journal of Canadian Petroleum Technology, 36(9). https://doi.org/10.2118/97-09-07
Gat, D., Tsesarsky, M., Shamir, D., & Ronen, Z. (2014). Accelerated microbial-induced CaCO₃ precipitation in a defined coculture of ureolytic and non-ureolytic bacteria. Biogeosciences, 11, 2561–2569. https://doi.org/10.5194/bg-11-2561-2014
Jiang, N.-J., Soga, K., & Kuo, M. (2017). Microbially induced carbonate precipitation for seepage-induced internal erosion control in sand–clay mixtures. Journal of Geotechnical and Geoenvironmental Engineering, 143(3), 04016100.
Kim, G., & Youn, H. (2016). Microbially induced calcite precipitation employing environmental isolates. Materials, 9(6), 468. https://doi.org/10.3390/ma9060468
Lo Bianco, A., & Madonia, G. (2007). B.U.L.M. technique for increasing the bearing capacity in pavement layers subjected to biological treatment. 4th International SIIV Congress, University of Palermo, Italy.
Mayur, S. V., & Jayeskumar, P. (2013). Bacterial concrete: A new era for the construction industry. International Journal of Engineering Trends and Technology, 4(9), 4128–4137.
Murtala, U., Khairul, A. K., & Kenny, T. P. C. (2016). Biological process of soil improvement in civil engineering: A review. Journal of Rock Mechanics and Geotechnical Engineering, 8, 767–774.
Neupane, S. (2016). Evaluating the Suitability of Microbial Induced Calcite Precipitation Technique for Stabilizing Expansive Soils (MSc thesis). Boise State University.
Osinubi, K. J., Eberemu, A. O., Ijimdiya, S. T., Yakubu, S. E., & Sani, J. E. (2017). Potential use of Bacillus pumilus in microbial induced calcite precipitation improvement of lateritic soil. 2nd Symposium on Coupled Phenomena in Environmental Geotechnics (CPEG2), Leeds, UK.
Osinubi, K. J., Sani, J. E., Eberemu, A. O., Ijimdiya, T. S., & Yakubu, S. E. (2018). Unconfined compressive strength of Bacillus pumilus treated lateritic soil. 8th International Congress on Environmental Geotechnics, Vol. 3, 410–418.
Seifan, M., & Berenjian, A. (2019). Microbially induced calcium carbonate precipitation: A widespread phenomenon in the biological world. Applied Microbiology and Biotechnology, 103, 4693–4708.
Seifan, M., Sarabadani, Z., & Berenjian, A. (2020). Microbially induced calcium carbonate precipitation to design a new type of bio self-healing dental composite. Applied Microbiology and Biotechnology, 104, 2029–2037.
Soon, N. W., Lee, L. M., & Hii, S. L. (2012). An overview of the factors affecting microbial-induced calcite precipitation and its potential application in soil improvement. World Academy of Science, Engineering and Technology, 62, 723–729.
Stabnikov, V., Chu, J., Myo, A. N., & Ivanov, V. (2013). Immobilization of sand dust and associated pollutants using bioaggregation. Water, Air, & Soil Pollution, 224(9), 1631. https://doi.org/10.1007/s11270-013-1631-0
Stocks-Fischer, S., Galinat, J. K., & Bang, S. S. (1999). Microbiological precipitation of CaCO₃. Soil Biology and Biochemistry, 31(11), 1563–1571.
Sun-Gyu, C., Sung-Sik, P., Shifan, W., & Jian, C. (2017). Methods for calcium carbonate content measurement of biocemented soils. Journal of Materials in Civil Engineering, 29(11), 06017015. https://doi.org/10.1061/(ASCE)MT.1943-5533.0002064
Van Paassen, L. A. (2011). Bio-mediated ground improvement: From laboratory experiment to pilot applications. In Geo-Frontiers: Advances in Geotechnical Engineering (pp. 4099–4108). Dallas, TX, USA.
Wei, S., Cui, H., Jiang, Z., Liu, H., He, H., & Fang, N. (2015). Biomineralization processes of calcite induced by bacteria isolated from marine sediments. Brazilian Journal of Microbiology, 46(2), 455–464.
Whiffin, V. S., Van Paassen, L. A., & Harkes, M. P. (2007). Microbial carbonate precipitation as a soil improvement technique. Geomicrobiology Journal, 25(5), 417–423. https://doi.org/10.1080/01490450701436505
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