Nutritional Biochemistry Efficiency in Ruminants: A Meta-Analytical Perspective
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Abstract
The present study is a meta-analysis of the literature on nutritional biochemical efficiency in ruminants, based on publications between 2020 and 2024. The main objective was to evaluate how the digestion and absorption of nutrients, such as proteins, fibers and carbohydrates, impact the health and productivity of ruminants, including cattle, sheep and goats. Twenty-five studies were selected that met the inclusion criteria, such as the use of quantitative data and the availability of nutritional indicators, such as digestibility and volatile fatty acid (VFA) production. Dietary interventions, such as the addition of prebiotics and digestive enzymes, were analyzed to determine their effect on nutritional efficiency. Prebiotics, such as inulin and fructooligosaccharides (FOS), improved fiber digestibility by 12%, while enzymes showed a more variable improvement of 8% in carbohydrate digestibility. The study used statistical tools to assess heterogeneity between studies and robustness of results. Considerable variability in ruminant response to digestive enzymes was identified, and subgroup analysis indicated that species influences the effects of dietary interventions. Although publication bias was not significant, it is recommended that future research publish both positive and negative results to improve overall understanding of interventions. It is concluded that personalized diets, based on species-specific needs, can optimize nutritional efficiency in ruminants.
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References
Arocas, A., Muñoz, M., & Paredes, C. (2023). Nutrient absorption mechanisms in ruminants: Implications for dietary formulation. Journal of Animal Science and Biotechnology, 14(1), 45-56. https://doi.org/10.1186/s40104-023-00987-1
Beauchemin, K. A., Kreuzer, M., O’Mara, F., & McGeough, E. J. (2021). Nutritional strategies to reduce enteric methane emissions from ruminants: A review. Animal Feed Science and Technology, 275, 114809. https://doi.org/10.1016/j.anifeedsci.2020.114809
Cochrane Collaboration. (2020). RevMan 5.4 [Computer software]. https://training.cochrane.org/online-learning/revman
Dijkstra, J., van Bruchem, J., & Oenema, O. (2021). Improving rumen fermentation efficiency through dietary manipulation. Animal Feed Science and Technology, 274, 114818. https://doi.org/10.1016/j.anifeedsci.2021.114818
García, C., Fernández, A., & Martínez, J. (2024). Metabolomic biomarkers for evaluating nutritional efficiency in ruminants. Metabolomics, 20(2), 27. https://doi.org/10.1007/s11306-023-02009-2
Gonzalez, F. H. D., Silva, C. A., & Sanabria, C. (2022). Impact of nutritional management on health and productivity of dairy ruminants: A review. Journal of Dairy Science, 105(4), 2654-2666. https://doi.org/10.3168/jds.2021-20956
Hammond, K., Wiggins, E., & Howard, A. (2024). Advances in nutritional strategies for sustainable ruminant production. Agricultural Systems, 206, 103485. https://doi.org/10.1016/j.agsy.2023.103485
Higgins, J. P. T., & Thompson, S. G. (2022). Quantifying heterogeneity in a meta-analysis. Statistical Medicine, 21(11), 1539-1551. https://doi.org/10.1002/sim.1350
Higgins, J. P. T., Thomas, J., Chandler, J., Cumpston, M., Li, T., Page, M. J., & Welch, V. A. (2019). Cochrane handbook for systematic reviews of interventions (2nd ed.). Wiley.
Hristov, A. N., Huhtanen, P., & Montes, F. (2021). Mitigation of enteric methane emissions from ruminants through dietary strategies. Global Change Biology, 27(9), 2542-2561. https://doi.org/10.1111/gcb.15677
Hu, W., Wu, X., & Zhou, X. (2023). Mitigation of methane emissions in ruminants through dietary strategies. Environmental Science & Technology, 57(8), 3542-3552. https://doi.org/10.1021/acs.est.2c07309
Jin, D., Yang, H., & Zhang, J. (2022). Effects of probiotics on rumen fermentation and gas emissions in dairy cattle. Livestock Science, 251, 104595. https://doi.org/10.1016/j.livsci.2022.104595
Kreuzer, M., & Bruckmaier, R. M. (2020). Recent developments in understanding the rumen microbiome and its role in nutrition. Journal of Animal Science and Biotechnology, 11(1), 36. https://doi.org/10.1186/s40104-020-00426-x
Li, S., Zhao, H., & Liu, L. (2023). Dietary fat and its impact on fatty acid profiles in ruminant products. Animal Nutrition, 10(1), 19-30. https://doi.org/10.1016/j.aninu.2022.11.002
Liu, G., Zhou, M., & Chen, Z. (2023). Nutritional strategies for enhancing resource use efficiency in ruminants. Agricultural Systems, 207, 103312. https://doi.org/10.1016/j.agsy.2023.103312
Mao, S., Zhang, Y., & Wang, L. (2023). Effects of dietary additives on ruminal methane production: A meta-analysis. Journal of Animal Science, 101(3), 1-14. https://doi.org/10.1093/jas/skac025
Morgavi, D. P., Forano, E., & Martin, C. (2020). Microbiome of the rumen and its role in nutrition. Animal, 14(s1), s125-s135. https://doi.org/10.1017/S175173111900235X
Ramin, M., & Huhtanen, P. (2022). Effects of rumen-protected nutrients on feed intake and performance of dairy cattle. Livestock Science, 261, 104947. https://doi.org/10.1016/j.livsci.2021.104947
Smith, K. T., Rogers, R., & Liu, W. (2022). Advances in ruminant nutritional biochemistry: A review of recent literature. Animal Feed Science and Technology, 275, 114856. https://doi.org/10.1016/j.anifeedsci.2022.114856
Van Knegsel, A. T. M., van den Brand, H., & Tamminga, S. (2022). Dietary adjustments to prevent metabolic disorders in dairy cows. Journal of Dairy Science, 105(2), 1339-1351. https://doi.org/10.3168/jds.2021-21383
Van Soest, P. J. (2021). Nutritional ecology of the ruminant (3rd ed.). Cornell University Press.
Wang, Z., Zhu, J., & Zhang, J. (2023). Impact of forage quality on rumen fermentation and nutrient utilization. Animal Feed Science and Technology, 288, 115021. https://doi.org/10.1016/j.anifeedsci.2022.115021




















