Effects of AlCl3 on the Enzymatic Antioxidants of Wister Rats Treated with Moringa oleifera Seed Extracts
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10.58578/ajstm.v1i1.3693
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Abstract
Determination of Malondialdehyde, MDA in blood plasma or tissue homogenates is one of the useful methods to predict the oxidative stress levels. The current study investigates the ameliorative effects of the seed extracts of Moringa oleifera on 35 albino rats induced with AlCl3 toxicity. Biomarkers of oxidative stress (Superoxide Dismutase, SOD; Catalase, CAT; Glutathione Peroxidase, GPx and Malondialdehyde, MDA were assayed. The plant seed extracts were shown to reduce the levels of MDA increased by AlCl3. AlCl3 caused decrease in (glutathione peroxidase) GPx levels as it causes MDA to significantly get elevated. The results showed that GPx decreased from 9.48 ± 0.86 to 6.68 ± 1.73 but upon treatments with 100 mg/kg bw of M. oleifera, GPx levels increased to 8.84 ± 0.86 (ethanol) and 8.96 ± 0.86 (aqueous). Increasing the concentrations of the extracts further increased the GPx levels while MDA were reduced.
Keywords:
Moringa oleifera; Superoxide Dismutase; Catalase; Glutathione Peroxidase; Malondialdehyde
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Yusufu, D., Zinas, S. T., Habibu, B., Amen, A., Umaru, I. J., Ejeh, Y. O., Grace, O. N., Henry, O. A., Audu, A. M., & Umaru, K. I. (2024). Effects of AlCl3 on the Enzymatic Antioxidants of Wister Rats Treated with Moringa oleifera Seed Extracts. African Journal of Sciences and Traditional Medicine, 1(1), 405-416. https://doi.org/10.58578/ajstm.v1i1.3693
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Hosny, E.N., Sawie, H.G., Elhadidy, M.E. and Khadrawy, Y.A. (2018). eval_uation of antioxidant and anti-inflammatory efficacy of caffeine in rat model of neurotoxicity. Nutritional Neuroscience. 1-8.
Mirshafa, A., Nazari, M., Jahani, D. and Shaki, F. (2018). Size-dependent neurotoxicity of aluminium oxide particles: a comparison between nano-and micrometer size on the basis of mitochondrial oxidative damage. Biological Trace Element Research. 1-9.
Zaky, A., Bassiouny, A., Farghaly, M. and Elsabaa, B.M. (2017). A Combination of Resveratrol and Curcumin is Effective Against Aluminium Chloride-Induced Neuroinflammation in Rats. Journal of Alzheimer's Disease. 60: 221-235.
Waterman, C., Cheng, D. M., Rojas-Silva, P., Poulev, A., Dreifus, J., Lila, M. A. and Raskin, I. (2014). Stable, water extractable isothiocyanates from Moringa oleifera leaves attenuate inflammation in vitro. Phytochemistry. 103: 114–122.
Ndhlala, A.R., Mulaudzi, R., Ncube, B., Abdelgadir, H.A., du Plooy, C.P. and Van Staden, J. (2014). Antioxidant, antimicrobial and phytochemical variations in thirteen Moringa oleifera Lam. cultivars. Molecules. 19 (7): 10480–10494.
Kushwaha, S., Chawla, P. and Kochhar, A. (2014). Effect of supplementation of drumstick (Moringa oleifera) and amaranth (Amaranthus tricolor) leaves powder on antioxidant profile and oxidative status among postmenopausal women. J Food Sci. Technol. 51 (11): 3464–3469.
Galuppo, M., Giacoppo, S., De Nicola, G.R., Iori, R., Navarra, M., Lombardo, G.E., Bramanti, P. and Mazzon, E. (2014). Antiinflammatory activity of glucomoringin isothiocyanate in a mouse model of experimental autoimmune encephalomyelitis. Fitoterapia. 95: 160–174.
Pandey, G. and Jain, G.C. (2013). A review on toxic effects of aluminium exposure on male reproductive system and probable mechanisms of toxicity. Int. J Toxicol & Appl. Pharmacol. 3(3): 48-57.
Geyikoglu, F., Turkez, H., Bakir, T.O. and Cicek, M. (2012). The genotoxic, hepatotoxic, nephrotoxic, haematotoxic and histopathological effects in rats after aluminium chronic intoxication. Toxicology and Industrial Health.
Buraimoh, A.A., Ojo, S.A., Hambolu, J.O. and Adebisi, S.S. (2012) Histological study of the effects of aluminium chloride exposure on the testis of Wistar rats. Am. Int. J Contem. Res. 2: 114–122.
Yousef, M.I. and Salama, A.F. (2009). Propolis protection from reproductive toxicity caused by aluminium chloride in male rats. Food Chem Toxicol. 47: 1168 – 1175.
Guo, C.H., Lin, C.Y., Yeh, M.S. and Hsu, G.S.W. (2005). Aluminum-induced suppression of testosterone through nitric oxide production in male mice. Envr. Toxicol Pharmacol. 19: 33–40.
Chinoy, N.J., Momin, R. and Jhala, D.D. (2005). Fluoride and aluminium induced toxicity in mice epididymis and its mitigation by vitamin C. Fluoride. 38: 115–121.
Keshari, A.K., Verma, A.K., Kumar, T. and Srivastava, R. (2015). Oxidative Stress: A Review. Int. J. Sci. & Tech. 3(7): 155–162.
Keshari, A.K. and Farooqi, H. (2014). eval_uation of the effect of hydrogen peroxide (H2O2) on haemoglobin and the protective effect of glycine. Int. J. Sci. & Tech. 2(2): 73–99.
Kumari, S., Verma, A.K., Rungta, S., Mitra, R., Srivastava, R. and Kumar, N. (2013). Serum Prolidase Activity, Oxidant and Antioxidant Status in Non-ulcer Dyspepsia and Healthy Volunteers. ISRN Biochem. 2013: 182601-182606.
Verma, A.K., Chandra, S., Singh, R.G., Singh, T.B., Srivastava, S. and Srivastava, R. (2014). Serum Prolidase Activity and Oxidative Stress in Diabetic Nephropathy and End Stage Renal Disease: A Correlative Study with Glucose and Creatinine. Biochem. Res. Int. 14: 291-297.
Fridovich, I. (1989). Superoxide radical and superoxide dismutases. Ann Rev. of Biochem. 64: 97–112.
Sinha, K.A. (1972). Colorimetric Assay of Catalase. Annals of Biochemistry. 47: 389 – 394.
Fahal, E. M., Rani, A.M.B., Aklakur, M.D., Chanu, T.I. and Saharan, N. (2018). Qualitative and Quantitative Phytochemical Analysis of Moringa oleifera (Lam) Pods. International Journal of Current Microbiology and Applied Sciences. 7(5): 657 – 665.
Khan, A., Suleman, M., Baqi, A., Ayub, S. and Ayub, M. (2018). Phytochemicals and their role in curing fatal diseases: A Review. Pure and Applied Biology. 8(1): 343 – 354.
Khafaga, A.F. and Bayad, A.E. (2016). Ginkgo biloba extract attenuates hematological disorders, oxidative stress and nephrotoxicity induced by single or repeated injection cycles of cisplatin in rats: Physiological and Pathological Studies. Asian Journal of Animal Science. 10: 235–246.
Khafaga, A.F. (2017). Exogenous phosphatidylcholine supplementation retrieve aluminum-induced toxicity in male albino rats. Environmental Science Poll Research. 24: 15589–15598.
Turgut, G., Enli, Y., Kaptanoğlu, B., Turgut, S. and Genç, O. (2006). Changes in the levels of MDA and GSH in mice. Eastern Journal of Medicine. 11: 7-12.
Hosny, E.N., Sawie, H.G., Elhadidy, M.E. and Khadrawy, Y.A. (2018). eval_uation of antioxidant and anti-inflammatory efficacy of caffeine in rat model of neurotoxicity. Nutritional Neuroscience. 1-8.
Mirshafa, A., Nazari, M., Jahani, D. and Shaki, F. (2018). Size-dependent neurotoxicity of aluminium oxide particles: a comparison between nano-and micrometer size on the basis of mitochondrial oxidative damage. Biological Trace Element Research. 1-9.
Zaky, A., Bassiouny, A., Farghaly, M. and Elsabaa, B.M. (2017). A Combination of Resveratrol and Curcumin is Effective Against Aluminium Chloride-Induced Neuroinflammation in Rats. Journal of Alzheimer's Disease. 60: 221-235.
