Antibacterial Effect of Methanol Extract of Newbouldia laevis Leaves on Some Selected Resistant Pathogen on Synthesised Drugs
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10.58578/kijst.v1i1.3399
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Abstract
Newbouldia laevis is a tropical plant belonging to the family of Bignoniaceae. It is among the most useful plants in Africa. Historically medicinal plants have been provided a good source of inspiration for novel therapeutic drugs which has made a large contribution to health and well-being of humans. It has been used over the years to as curative agents against many infections and have been exploited in the traditional medicine with their curative potentials. Material and methods: The leaves of Newbouldia laevis was bought from a local market in Wukari, Taraba State. The leaves of Newbouldia laevis was chop into pieces, air-dried for four days and then pulverized into fine powder. About 250 g of the powdered bark extracted with 2 L of ethanol using maceration method for 72 hrs. The crude extracts of Newbouldia laevis was used in antibacterial assay. The results observed demonstrate that the Newbouldia leaves methanol crude extract possesses concentration-dependent antibacterial activity against both Gram-positive resistant bacteria (Staphylococcus aureus) and Gram-negative (Escherichia coli, Klebsiella pneumonia) bacteria. The increasing inhibition zones with higher concentrations suggest a potential dose-response relationship.
Keywords:
Antibacterial; Methanol; Extract; Newbouldia Laevis; Leaves; Resistant Pathogen; Synthesised; Drugs
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Umaru, K. I., Ugoeze, E. S., Tansaba, A. A., Nanmar, C., Danjuma, T. L., & Umaru, I. J. (2024). Antibacterial Effect of Methanol Extract of Newbouldia laevis Leaves on Some Selected Resistant Pathogen on Synthesised Drugs. Kwaghe International Journal of Sciences and Technology, 1(1), 64-75. https://doi.org/10.58578/kijst.v1i1.3399
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References
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Germann K, Kaloga M, Ferreira D, Marais JP, Kolodziej H. (2006). Newbouldioside A-C Phenylethanoid Glycosides from the stembark of Newbouldia leavis. Phytochem; 67(8): 805–811Edwards R, Harding KG. (2004). Bacteria and wound healing. Curr Opin Infect Dis; 17(2): 91- 96.
Usman H, Osuji JC. (2007). Phytochemical and in vitro antimicrobial assay of the leaf extract of Newbouldia laevis. African Journal of Tradit. Complement. Altern. Med. 4(4):476-480.
Anton CD, Andries GG, Hermien G. (1996). Common eye disorders. In: Eric Herfindal T, Dick Gourlry R, editors. Textbook of Therapeutics: Drug and Disease Management, 6th edition, Williams and Wilkins, USA, p 937
Al-Bayati FA, Al-Mola HF. (2008). Antibacterial and antifungal activities of different parts of Tribulus terrestris L. growing in Iraq. J Zhejiang Univ. Sci.; 9(2): 154–159
Agbafor KN, Ezeali C, Akubugwo EI, Obiudu IK, Uraku AJ, Ogbanshi ME. (2015). Cardioprotective effect of leaf and root extracts of Newbouldia laevis against carbon tetrachloride induced-cardiotoxicity in albino rats. Eur J Med Plants.;9(3):1-7. doi:10.9734/ EJMP/2015/17673
Burkill H. (1997). The Useful Plants of West Tropical Africa. 2nd ed. Vol. 4 (Families M-R), Kew: Royal Botanic Gardens; 1997.
Fifa T. D. Bothon, Moutcharaf Moustapha, G. Sophie Bogninou, C. Pascal Agbangnan Dossa, Boniface Yehouenou, Seindé E. Medoatinsa, Jean Pierre Noudogbessi, Félicien Avlessi and Dominique C. K. Sohounhloue. (2014). Chemical Characterization and Biological Activities of Newbouldia laevis and Pterocarpus Santalinoides Leaves. Bull. Env. Pharmacol. Life Sci., 3 [11] 09-15
Fatunla AO, Ogundare OA, Achimugu II, Akindele PO. (2018). Antibacterial effect of Newbouldia laevis leaf extract on vancomycin and methicillin resistant Bacterial Isolates from Federal Medical Center, Owo. JAMB.;1(3):1-11.
Anaduaka EG, Ogugua VN, Egba S, Apeh VO. (2013). Investigation of some important phytochemical, nutritional properties and toxicological potentials of ethanol extracts of Newbouldia laevis leaf and stem. African Journal of Biotechnology.;12(40): 5846-5949.
Azando EV, Hounzangbe-Adote MS, Olounlade PA, Brunet S, Fabre N, Valentin A, Hoste H. (2011). Involvement of tannins and flavonoids in the in vitro effects of Newbouldia laevis and Zanthoxylum zanthoxyloides extracts on the exsheathment of third-stage infective larvae of gastrointestinal nematodes. Vet. Parasitol.;180(3-4):292-297.
Innocent U. Okagu, Joseph C. Ndefo, Matthias O. Agbo. (2022). Trado-Medical Uses, Chemical Constituents and Biological Activities of Newbouldia laevis (Bignoniaceae): A Review. Pharmaceutical Sciences, , 28(1), 51-75 doi:10.34172/PS.2021.29
Dermane A, Kpegba K, Eloh K, Osei-Safo D, Amewu RK, Caboni P. (2020). Differential constituents in roots, stems and leaves of Newbouldia laevis Thunb. screened by LC/ESIQ-TOF-MS. Results Chem.; 2:100052
Kolodziej H. (2021). Studies on Bignoniaceae: newbouldiosides D–F, minor phenylethanoid glycosides from Newbouldia laevis, and new flavonoids from Markhamia zanzibarica and Spathodea campanulata. Planta Med. 87(12-13):989-97 doi:10.1055/a-1270-7761
Gormann R, Kaloga M, Li XC, Ferreira D, Bergenthal D, Kolodziej H. Furanonaphthoquinones, atraric acid and a benzofuran from the stem barks of Newbouldia laevis. Phytochemistry. 2003;64:583-87
Hassan SW, Salawu K, Ladan MJ, Hassan LG, Umar RA, Fatihu MY. Hepatoprotective, antioxidant and phytochemical properties of leaf extracts of Newbouldia laevis. Int J PharmTech Res. 2010;2(1):573-84.
Enye LA, Edem EE, Gonaka MG, Ekundina V, Akingbade AM, Adefisayo MA. (2015). The effect of Newbouldia laevis on wistar rat’s testis and liver histomorphology in cadmium-induced testicular toxicity and hepatotoxicity. World J Pharm Pharm Sci.;4(11):100-08.
Itez P, Aloh GS, Osuocha KU, Iwueke AV, Chukwu EC. (2020). Ameliorative potentials of methanol leaf extract of Newbouldia laevis on monosodium glutamate induced toxicity in female albino rats. Afr J Bio Sci. 2020;2(3):37-50. doi:10.33472/AFJBS.2.3.2020.37-50
Andrede-Cetto A, Heinrich M. (2005). Mexican plants with hypoglycaemic effect used in the treatment of diabetes. J Ethnopharmacol.;99(3):325-48.
Bosha JA, Sule S, Asuzu IU. (2019). Antidiabetic and hypolipidemic effects of methanol leaf extract of Newbouldia laevis in alloxan induced diabetic rats. Adv Res Gastroentero Hepatol.;13(3):56-62. doi:10.19080/ARGH.2019.13.555863
Kalakonda A, Jenkins BA, John S. Physiology, Bilirubin (2022). In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2024 Jan-. Available from: https://www.ncbi.nlm.nih.gov/books/NBK470290/
Mbagwu IS, Akah PA, Ajaghaku DL, Ike JC, Okoye FBC. Newboulasides A and B, two new caffeic acid glycosides from Newbouldia laevis with α-amylase inhibitory activity. Nat Prod Res. 2020. doi:10.1080/1 4786419.2020.1799362
Osigwe CC, Akah PA, Nworu CS, Okoye FBC. (2017). Apigenin: A methanol fraction component of Newbouldia laevis leaf, as a potential antidiabetic agent. J Phytopharmacol. 6(1):38-44.
Rauter AP, Martins A, Borges C, Mota-Filipe II, Pinto R, Sepodes B, et al. (2010). Antihyperglycemic and protective effects of flavonoids on streptozotocin-induced diabetic rats. Phytother Res. 2010; 24: S133-38.
Murtala AA, Akindele AJ. (2020). Anxiolytic- and antidepressant-like activities of hydroethanol leaf extract of Newbouldia laevis (P. Beauv.) Seem. (Bignoniaceae) in mice. J Ethnopharmacol. 249:112420. doi: 10.1016/j.jep.2019.112420
Bosha JA, Anaga AO, Asuzu IU. (2015). Bioassay-guided isolation and structural elucidation of antidiabetic principle of methanol leaf extract of Newbouldia laevis (P Beauv). J Pharm Pharmacol.;3:515-26.
Umaru, I. J., & Samling, B. (2018). Determining the antibacterial effect of Leptadenia hastata extracts against Klebsiella Pneumonia and staphylococcus aureus. MOJ Proteomics Bioinformatics, 7(6), 176-178.
Kolodziej H. (2021). Studies on Bignoniaceae: newbouldiosides D–F, minor phenylethanoid glycosides from Newbouldia laevis, and new flavonoids from Markhamia zanzibarica and Spathodea campanulata. Planta Med. 87(12-13):989-97 doi:10.1055/a-1270-7761
Kuete V, Eyong KO, Folefoc GN, Beng VP, Hussain H, Krohn K, et al. (2007). Antimicrobial activity of the methanolic extract and of the chemical constituents isolated from Newbouldia laevis. Pharmazie. 62:552-56. doi:10.1691/ph.2007.7.6757
Akerele, J. O., Ayinde, B. A., and Ngiagah, J. (2011). Phytochemical and Antibacterial eval__uations of the Stem Bark of Newbouldia laevis against Isolates from Infected Wounds and Eyes, Tropical Journal of Pharmaceutical Research. 10 (2): 211-217.
Germann K, Kaloga M, Ferreira D, Marais JP, Kolodziej H. (2006). Newbouldioside A-C Phenylethanoid Glycosides from the stembark of Newbouldia leavis. Phytochem; 67(8): 805–811Edwards R, Harding KG. (2004). Bacteria and wound healing. Curr Opin Infect Dis; 17(2): 91- 96.
Usman H, Osuji JC. (2007). Phytochemical and in vitro antimicrobial assay of the leaf extract of Newbouldia laevis. African Journal of Tradit. Complement. Altern. Med. 4(4):476-480.
Anton CD, Andries GG, Hermien G. (1996). Common eye disorders. In: Eric Herfindal T, Dick Gourlry R, editors. Textbook of Therapeutics: Drug and Disease Management, 6th edition, Williams and Wilkins, USA, p 937
Al-Bayati FA, Al-Mola HF. (2008). Antibacterial and antifungal activities of different parts of Tribulus terrestris L. growing in Iraq. J Zhejiang Univ. Sci.; 9(2): 154–159
Agbafor KN, Ezeali C, Akubugwo EI, Obiudu IK, Uraku AJ, Ogbanshi ME. (2015). Cardioprotective effect of leaf and root extracts of Newbouldia laevis against carbon tetrachloride induced-cardiotoxicity in albino rats. Eur J Med Plants.;9(3):1-7. doi:10.9734/ EJMP/2015/17673
Burkill H. (1997). The Useful Plants of West Tropical Africa. 2nd ed. Vol. 4 (Families M-R), Kew: Royal Botanic Gardens; 1997.
Fifa T. D. Bothon, Moutcharaf Moustapha, G. Sophie Bogninou, C. Pascal Agbangnan Dossa, Boniface Yehouenou, Seindé E. Medoatinsa, Jean Pierre Noudogbessi, Félicien Avlessi and Dominique C. K. Sohounhloue. (2014). Chemical Characterization and Biological Activities of Newbouldia laevis and Pterocarpus Santalinoides Leaves. Bull. Env. Pharmacol. Life Sci., 3 [11] 09-15
Fatunla AO, Ogundare OA, Achimugu II, Akindele PO. (2018). Antibacterial effect of Newbouldia laevis leaf extract on vancomycin and methicillin resistant Bacterial Isolates from Federal Medical Center, Owo. JAMB.;1(3):1-11.
Anaduaka EG, Ogugua VN, Egba S, Apeh VO. (2013). Investigation of some important phytochemical, nutritional properties and toxicological potentials of ethanol extracts of Newbouldia laevis leaf and stem. African Journal of Biotechnology.;12(40): 5846-5949.
Azando EV, Hounzangbe-Adote MS, Olounlade PA, Brunet S, Fabre N, Valentin A, Hoste H. (2011). Involvement of tannins and flavonoids in the in vitro effects of Newbouldia laevis and Zanthoxylum zanthoxyloides extracts on the exsheathment of third-stage infective larvae of gastrointestinal nematodes. Vet. Parasitol.;180(3-4):292-297.
Innocent U. Okagu, Joseph C. Ndefo, Matthias O. Agbo. (2022). Trado-Medical Uses, Chemical Constituents and Biological Activities of Newbouldia laevis (Bignoniaceae): A Review. Pharmaceutical Sciences, , 28(1), 51-75 doi:10.34172/PS.2021.29
Dermane A, Kpegba K, Eloh K, Osei-Safo D, Amewu RK, Caboni P. (2020). Differential constituents in roots, stems and leaves of Newbouldia laevis Thunb. screened by LC/ESIQ-TOF-MS. Results Chem.; 2:100052
Kolodziej H. (2021). Studies on Bignoniaceae: newbouldiosides D–F, minor phenylethanoid glycosides from Newbouldia laevis, and new flavonoids from Markhamia zanzibarica and Spathodea campanulata. Planta Med. 87(12-13):989-97 doi:10.1055/a-1270-7761
Gormann R, Kaloga M, Li XC, Ferreira D, Bergenthal D, Kolodziej H. Furanonaphthoquinones, atraric acid and a benzofuran from the stem barks of Newbouldia laevis. Phytochemistry. 2003;64:583-87
Hassan SW, Salawu K, Ladan MJ, Hassan LG, Umar RA, Fatihu MY. Hepatoprotective, antioxidant and phytochemical properties of leaf extracts of Newbouldia laevis. Int J PharmTech Res. 2010;2(1):573-84.
Enye LA, Edem EE, Gonaka MG, Ekundina V, Akingbade AM, Adefisayo MA. (2015). The effect of Newbouldia laevis on wistar rat’s testis and liver histomorphology in cadmium-induced testicular toxicity and hepatotoxicity. World J Pharm Pharm Sci.;4(11):100-08.
Itez P, Aloh GS, Osuocha KU, Iwueke AV, Chukwu EC. (2020). Ameliorative potentials of methanol leaf extract of Newbouldia laevis on monosodium glutamate induced toxicity in female albino rats. Afr J Bio Sci. 2020;2(3):37-50. doi:10.33472/AFJBS.2.3.2020.37-50
Andrede-Cetto A, Heinrich M. (2005). Mexican plants with hypoglycaemic effect used in the treatment of diabetes. J Ethnopharmacol.;99(3):325-48.
Bosha JA, Sule S, Asuzu IU. (2019). Antidiabetic and hypolipidemic effects of methanol leaf extract of Newbouldia laevis in alloxan induced diabetic rats. Adv Res Gastroentero Hepatol.;13(3):56-62. doi:10.19080/ARGH.2019.13.555863
Kalakonda A, Jenkins BA, John S. Physiology, Bilirubin (2022). In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2024 Jan-. Available from: https://www.ncbi.nlm.nih.gov/books/NBK470290/
Mbagwu IS, Akah PA, Ajaghaku DL, Ike JC, Okoye FBC. Newboulasides A and B, two new caffeic acid glycosides from Newbouldia laevis with α-amylase inhibitory activity. Nat Prod Res. 2020. doi:10.1080/1 4786419.2020.1799362
Osigwe CC, Akah PA, Nworu CS, Okoye FBC. (2017). Apigenin: A methanol fraction component of Newbouldia laevis leaf, as a potential antidiabetic agent. J Phytopharmacol. 6(1):38-44.
Rauter AP, Martins A, Borges C, Mota-Filipe II, Pinto R, Sepodes B, et al. (2010). Antihyperglycemic and protective effects of flavonoids on streptozotocin-induced diabetic rats. Phytother Res. 2010; 24: S133-38.
Murtala AA, Akindele AJ. (2020). Anxiolytic- and antidepressant-like activities of hydroethanol leaf extract of Newbouldia laevis (P. Beauv.) Seem. (Bignoniaceae) in mice. J Ethnopharmacol. 249:112420. doi: 10.1016/j.jep.2019.112420
Bosha JA, Anaga AO, Asuzu IU. (2015). Bioassay-guided isolation and structural elucidation of antidiabetic principle of methanol leaf extract of Newbouldia laevis (P Beauv). J Pharm Pharmacol.;3:515-26.
Umaru, I. J., & Samling, B. (2018). Determining the antibacterial effect of Leptadenia hastata extracts against Klebsiella Pneumonia and staphylococcus aureus. MOJ Proteomics Bioinformatics, 7(6), 176-178.
Kolodziej H. (2021). Studies on Bignoniaceae: newbouldiosides D–F, minor phenylethanoid glycosides from Newbouldia laevis, and new flavonoids from Markhamia zanzibarica and Spathodea campanulata. Planta Med. 87(12-13):989-97 doi:10.1055/a-1270-7761
Kuete V, Eyong KO, Folefoc GN, Beng VP, Hussain H, Krohn K, et al. (2007). Antimicrobial activity of the methanolic extract and of the chemical constituents isolated from Newbouldia laevis. Pharmazie. 62:552-56. doi:10.1691/ph.2007.7.6757
