Proximate and Phytochemical Constituents of Ethanolic Extract of Seeds of Cola Acuminata and Cola Nitida
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Abstract
This study evaluated the proximate and phytochemical constituents of ethanolic seeds extracts of Cola nitida and Cola acuminata. The seeds of C. acuminata and C. nitida were purchased and air-dried at room temperature. The dried seeds were pulverized to ground sample. The sample was weighed and used for the proximate and phytochemical analysis. The qualitative phytochemical screening revealed the presence of secondary metabolites: alkaloid, flavonoids, tannins, phenols, Cardiac glycoside, terpenes (tri), steroids and saponins, but the absence of anthraquinones. The seed of C. nitida was higher in alkaloids, saponins, phenols, tannins, steroids, triterpenes and C-glycosides than the seed of C. acuminata, while the seed of C. acuminata was higher in flavonoids and carbohydrates than the seed of C. nitida. There was no statistical significant difference (p>0.05) between the levels of alkaloids, saponins, phenols and tannins in seed of C. nitida and seed of C. acuminata. The difference between the levels of flavonoids, carbohydrates, steroids, triterpenes and C-glycosides in seed of C. nitida and seed of C. acuminata was statistically significant (p<0.05). The seed of C. nitida was higher in moisture, ash, lipid and crude protein than the seed of C. acuminata, while the seed of C. acuminata was higher in crude fibre and carbohydrate than the seed of C. nitida. There was no statistically significant difference (p>0.05) between the levels of moisture, ash, lipid, crude fibre and crude protein in seed of C. nitida and seed of C. acuminata. The difference between the level of carbohydrate in seed of C. nitida and seed of C. acuminata was statistically significant (p<0.05).
The result shows that seeds of C. acuminata and C. nitida possess an appreciable level of phytochemicals. It could be a good raw material for the production of some medicinal drugs and can be used in folk medicine for the treatment of some diseases.
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References
Adeleke, R.O. and Abiodun, O.A. (2010). Chemical composition of three traditional vegetables in Nigeria. Pakistan Journal Nutrition, 9 (9): 858– 860.
Adenuga, O.O., Oduwaye, O.F. and Adejobi, K. B. (2012). Incidence of mycoflora complexes on retail kolanuts (C. nitida and C. acuminata) in North Central Nigeria. Journal of Applied Biosciences, 56: 4075– 4079.
Adisa, W.A., Otamere, H.O., Osifo, C.U., Idonije, O.B. and Nwoke, E.O. (2010). Effects of Aqueous Extract of Kola Nut (Cola Nitida Rubra) on Reproductive Hormones in Rats. Nigeria Journal of Physiology Science. 25:121-123.
Ajai, A.I., Ochigbo S.S., Jacob, J.O., Ndamitso, M.M. and Abubakar, U. (2012). Proximate and Mineral Compositions of Different Species of Kola Nuts. European Journal of Applied Engrineering Science Research, 1: 44-47.
AOAC (Association of official analytical chemists), (2003). Official methods of analysis of the association of official’s analytical chemists, 17th edn, Arlington, Virginia.
Apiamu, A., Asagba, S. O. and Tonukari, N. J. (2018). Reporting the toxicological profile of methanolic leaf extract of Anthocleista vogelii planch on Wistar rats. Journal of Applied Science and Environmental Management, 22 (6): 887- 894.
Atanda, O. O., Olutayo, A., Mokwunye, F. C., Oyebanji, A. O. and Adegunwa, M. O. (2011). The Quality of Nigerian Kola Nuts. African Journal of Food Science, 5: 904-909.
Atiba, F. A., Fatokun, A. A., Imosemi, I. O., Malomo, A. O. (2021). Kola nut from Cola nitida vent. Schott administered to pregnant rats induces histological alterations in pups' cerebellum. PLoS One. 8;16(3):e0247573.
Attah, E. I., Ugwuagbo, S. C., Chinnam, S., Eze, F. I., Nnadi, C.O., Agbo, M.O., Obonga, W., Rudrapal, M., Walode, S. G., Nizam, A., Sahoo, R. K., Bendale, A. R., Khairnar, S. J and Jagtap, M. R. (2022). Anti-inflammatory Activity of Sabicea brevipes Wernharm (Rubiaceae). Pharmacia, 69 (2): 311–317.
Brosnan, J. (2006). Inter-organ amino acid transport and its regulation. Journal of Nutrition, 133: 2068S–2072S.
Burdock, G. A. (2005). Kola Nut (Cola Nut). In: Fenaroli's Handbook of Flavor Ingredients, Burdock, G.A. (Ed.). CRC Press, Boca Raton. 5: 993-994.
Chidume, C. G., Osisioma, U. S., and Echem, S. O. (2015). The Symbolism of Kolanut in Igbo Cosmology: a re-examination. International Journal of Research, 51.
Dahanukar, S. A., Kulkarni, R. A. and Rege, N. N. (2000). Pharmacology of medicinal plants and natural products. International Journal of Pharmacology ; 32:81-118.
Dah-Nouvlessounon, D., Adjanohoun, A., Sina, H., Noumavo, P. A., Diarrasouba, N., Parkouda, C., Madodé, Y. E., Dicko, M. H. and Baba-Moussa, L. (2015). Nutritional and antinutrient composition of three kola nuts (Cola nitida, Cola accuminata and Garciinia kola) produced in Benin. Food and Nutrition Sciences, 6 (15): 1395-1407.
Dewole E. A., Dewumi D. F. A., Alabi J. Y. T and Adegoke A. (2013). Proximate and phytochemicals of Cola nitida and Cola acuminata. Pakistan Journal of Biological Science, 16: 1593-1596.
Ejimofor, C., F, Nwakoby, N., E, Oledibe, O., J, Mbaukwu, O., A and Afam-Ezeaku, C., E. (2023). Determination of Proximate and Phytochemical Composition of Three Species of Beans Sold in Uli. Asian Journal of Food Research and Nutrition, Volume 2, Issue 4, Page 418-429.
Ekalu, A. and Habila, J. D. (2020). Pharmacology and medicinal uses of Cola (Malvaceae) family: a review. Phytochemistry, volume 29, pages 2089- 2105
Esimone, C .O., Adikwu, M. U., Nworu, C. S, Okoye, F. B. C., Odimegwu, D .C. (2017). Adaptogenic potentials of Camellia sinensis leaves, Garcinia kola and Kola nitida seeds. Scientific Research and Essays, 2 (7): 232-237.
Evuen, U. F., Okolie, N. P. and Apiamu, A. (2022). Evaluation of the mineral composition, phytochemical and proximate constituents of three culinary spices in Nigeria: a comparative study. Scientific Reports, 12: 20705.
Heinrich, M., Mah, J., and Amirkia, V. (2021). Alkaloids used as medicines: Structural phytochemistry meets biodiversity—an update and forward look. Molecules, 26(7), 1836.
Himal, P. C., Nisha, S. Y., Jyoiei, S., Anupa, K. C., Mansoor, S. and Panna, T. (2008). Phytochemical and antimicrobial evaluations of some medicinal plants of Nepala Kathmandeunw. Journal of Science Engineering and Technology, 1(5): 49-54.
Ibe–diala, J. C. and Igwe, O. U. (2022). Phytochemical Composition and Antioxidant Activity Screening of Aqueous Leaves of Man-To-Man (Peperomia pellucida) Harvested from Umunomon Ihitteafoukwu in Imo State, South Eastern Nigeria. Journal Applied Science Environment Management, 26 (12) 2067-2074.
Imo, C. (2017). Phytochemical and proximate composition of parts of Euphorbia hyssopifolia. AASCIT Journal of Biology, 3: 30-33.
Imo, C., Ojochenemi, E. Y., Nkeiruka, G. I., Ifeoma S. U., Silas V. Tatah and Ogochukwu, J. O. (2018). Proximate, mineral and phytochemical composition of Piper guineense seeds and leaves. Journal of Biological Sciences, 18: 329-337.
Imo, C., Uhegbu, F. O., Ifeanacho, N. G., and Azubuike, N. C. (2015). Histological and hepatoprotective effect of ethanolic leaf extract of Gongronema latifolium Benth in acetaminophen-induced hepatic toxicity in male albino rats. International Journal of Preventive Medicine Research, 1: 217-226.
Irabor, E. E. I., Eze, P. N., Unuigbe, C. A., Imafidon, M. I., Omoruyi, U. and Emmanuel, V. (2023). Proximate Analysis and Mineral Determination of Calliandra surinamensis Seed. Journal of Chemical Society of Nigeria, 48(1).
Kanu, I. A. (2020). The Igbo African Kolanut as a type of Holy Communion: Towards the Management of Cultural Diversity. Villanova Journal of Science, Technology and Management, Vol 2: 1.
Khan, M. I., Adebayo, K. O., Osigbemhe, I. G., Maliki, M., Bolaji, A. M., Paul, E. and Edeeje, J. P. (2023). Comparative proximate composition, anti-nutritional analyses and anti-microbial screening of some Nigerian medicinal plants. FUDMA Journal of Sciences, 7(2): 159-163.
Khanbabaee, K and vanRee, T. (2001). Tannins: Classification and definition. Natural Product Report, 18(6), 641–649.
Khatuntseva, E. A., Men’shov, V. M., Shashkov, A. S., Tsvetkov, Y. E., Stepanenko, R. N., Vlasenko, R. Y. and Nifantiev, N. E. (2012). Triterpenoid saponins from the roots of Acanthophyllum gypsophiloides regel. Beilstein journal of organic chemistry, 8(1), 763-775.
Kumavath, R., Paul, S., Pavithran, H., Paul, M.K.; Ghosh, P., Barh, D., Azevedo, V. (2021). Emergence of Cardiac Glycosides as Potential Drugs: Current and Future Scope for Cancer Therapeutics. Biomolecules, 11, 1275.
Lateef, A. (2023). Cola nitida: Milestones in catalysis, biotechnology and nanotechnology for circular economy and sustainable development. Biocatalysis and Agricultural Biotechnology, Volume 53, Page 102856
Lowe, H.I.,Watson, C. T., Badal, S., Peart, P., Toyang, N. J., Bryant, J. (2014). Promising efficacy of Cola accuminata plant. A mini review. Advances in Biological chemistry, 4: 240-245.
Makkar HP, Siddhuraju P, Becker K. Methods in molecular biology: plant secondary metabolites. Totowa: Human Press; 2007. pp. 93–100.
Margaret, S.A., Ishmael, A.I., Winifred O.K. and Samuel, T.L. (2022). Post-harvest quality assessment of freshly harvested and processed kola nuts [Cola nitida (Vent.) Schott & Endl.] From selected growing regions in Ghana. Cogent Food & Agriculture, 8: 2054565
Mills, S., Stanton, C., Lane, J. A., Smith, G. J. and Ross, R. P. (2019). Precision nutrition and the microbiome, Part I: Current state of the science. Nutrients. 11(4): 923.
Moon, J. Y., Kim, H., Lee, D., Cho, M and Choi, H. (2010). Antioxidant and antiproliferative activities of kola species. Journal Food Chemistry, 121(2):429-436.
Muhammad, S. and Fatima, A. (2014) Studies on Phytochemical Evaluation and Antibacterial properties of Two Varieties of Kolanut (Cola nitida) in Nigeria. Journal of Biosciences and Medicines, 2, 37-42.
Nyadanu, D., Samuel, T. L., Jerome, A. D., Abraham, A. and Micheal, B. M. (2023). Heterosis patterns and sources of self-compatibility, cross-compatibility and key nut traits within single and double hybrid crosses of kola [Cola nitida (Vent) Schott and Endl.]. Scientific Report. 13, 8036.
Odo, O. N., Nwokeocha, I. and Ezegwu, D. (2023). 'He Who Brings Kola Brings Life': Communicating the Significance of Kolanut among Igbo People of Nigeria, India. Indiana Journal of Arts & Literature, 4(1), 24-29
Ogbonnia, S. O., Mbaka, G. O., Anyika, E. N., Emordi, E. J and Nwakakwa, N. (2011). An evaluation of acute and subchronic toxicities of a Nigerian polyherbal tea remedy. Pakistan Journal of Nutrition, 10(11):1022-1028
Oguntibeju, O. O. (2018). Medicinal plants with anti-inflammatory activities from selected countries and regions of Africa. Journal of Inflammation Research, 11: e307
Okeke, C.U., Chinelo, A.E., Chimezie, H.U., Chidozie, D.B. and Okwuchukwu, A. Comparative Phytochemical and Proximate Compositions of Cola acuminata (P. Beauv.) Schott and Cola nitida (Vent) Schott and Endl. Plant. Vol. 3, No. 3, 2015, pp. 26-29.
Okunade, A., Agboola, T., Ogunsina, B., Salau, A. and Salami, A. (2019). Physicochemical properties of Four Selected Food Spices of Nigerian Origin: Alligator Pepper, Black Pepper, Ginger and Cloves. Ife Journal of Technology, 26(1), 7-12.
Oladeji, O. (2016). The characteristics and roles of medicinal plants: Some important medicinal plants in Nigeria. Natural Products: An Indian Journal, 12(3), 102–109.
Qiu, (2014). Natural alkaloids: Basic aspects, biological roles, and future perspectives. Chin. J. Nat. Med. 12(6), 401–406.
Salahdeen, H. M., Omoaghe, A. O., Isehunwa, G. O. and Murtala, B. A. (2014). Effects of chronic administrationof ethanolic extract of kola nut (Cola nitida) and caffeine on vascular function. African Journal of Medicine and Medical Sciences, 43:17-27.
Salawu,K., Owolarafe, T. A., Ononamadu, C. J., Ihegboro, G.O., Lawal, T. A., Aminu, M. A. and Oyekale, A. J. (2021). Phytochemical, Nutritional Composition and Heavy Metals Content of Allium cepa (Onion) and Allium sativum (Garlic) from Wudil Central Market, Kano State, Nigeria, Biokemistri Vol. 33, No. 4
Snehal, S. P. and Jignasha, K. S. (2015) Systematic review of plant steroids as potential antiinflammatory agents: Current status and future perspectives. The Journal of Phytopharmacology, 4(2): 121-125.
Sofowora, A. (1993). Phytochemical Screening of Medicinal Plants and Traditional Medicine in Africa, Spectrum Books Ltd, Ibadan, Nigeria.
Stray, F., 1998. The Natural guide to medicinal herbs and plants. Tiger Book International, London, pp: 12-16.
Trease, G. E. and Evans, W. C. (1999). “Phenols and phenolic glycosides,” in Textbook of Pharmacognosy, vol. 12, pp. 343–383, Balliese, Tindall and Co Publishers, London, UK.
Tsuchiya, (2017). Proximate analysis, Mineral composition, phytochemical contents, Antioxidant and Antimicrobial activities and GC-Ms investigation of various solvents extracts of cactus cladode. Food Science and Technology, 37(2).
Ullah, S., Munir, S., Badshah, S. L., Khan, N., Ghani, L., Poulson, B. G., and Jaremko, M. (2020). Important flavonoids and their role as a therapeutic agent. Molecules, 25(22), 5243.
Umoren, E., Osim, E.E. and Udoh, P.B. (2009). The comparative effects of chronic consumption of kolanut (Cola nitida) and caffeine diets on locomotor behaviour and body weight in mice. Nigerian journal of physiological sciences. 24(1):73-78.
Uttu, A.J., Waziri, M., Dauda, A. and Bida, K. M. (2021). A short review of medicinal plants extracts accompanied by potential antidepressant activity. Journal of Chemical Review, volume 3, issue 4, p. 307 - 319.
Uzoekwe, N.M and Ezenwajiugo, C.E. (2023). Phytochemicals, Elemental and Proximate analyses of Piper Guineense Leaves. Journal of Applied Science of Environment Management, 27 (4) 657-663
Vincken, J., Heng, L. and Gruppen, H. (2007). Saponins,classification and occurrence in the plant kingdom, Phytochemistry Volume 68, Issue 3, Pages 275-297.
