Effect of Ethanolic Stem Extract of Nelsonia Canescens on Selected Biochemical Parameters in Male Wistar Rats Induced with Sodium Arsenite
Please do not hesitate to contact us if you would like to obtain more information about the submission process or if you have further questions.
Abstract
Medicinal plants are those that have curative qualities or have positive pharmacological effects on the human body. The effect of ethanolic stem extracts from Nelsonia canescens was studied in relation to Sodium arsenite-induced toxicity in wistar rats. Fresh stem extract of Nelsonia canescens were obtained behind rice mill area, Wukari, Taraba state and was shade dried at room temperature and was homogenized into powder and measured at 300g into 100ml of absolute ethanol for 72 hours. 15 healthy male rats of 70g to 90g weight were obtained from animal house Makurdi, Benue state. Animals from Group 1 were used as control. 5mg/kg body weight of Sodium arsenite was administered to Group 2 animals while animals in Groups 3, 4 and 5 were administered with Nelsonia canescens ethanolic stem extracts 50 mg/kg, 100mg/kg and 200 mg/kg as well. At the end of 3 weeks the animals were sacrificed and serum sample were collected and analysed using standard methods. The results indicate that, when compared to those who received Sodium arsenite, those who received ethanol stem extracts of Nelsonia canescens showed a comparatively considerable liver protection against Sodium arsenite -induced damage. The levels of biochemical parameters: Albumin, Total protein, Alanine aminotransferase (ALT), Aspartate aminotransferase (AST), Alkaline phosphatase (ALP), Total bilirubin, Urea, Creatinine of rats administered with Sodium arsenite only was also observed. The Nelsonia canescens extract’s activity at 200mg/kg bw (higher dose) give a reasonable decrease in the amount of these liver enzymes. Deducing from study results, it indicates that Nelsonia canescens leaf extracts could be an effective agent in Sodium arsenite mediated liver toxicity in adult wistar rats and drug development.
Citation Metrics:
Downloads
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
References
Akinsiku, A.A, Ajanaku, K.O, Adekoya, J.A. and Dare, E.O. (2015). Green synthesis, characterization of silver nanoparticles using Canna indica and Senna occidental is leaf extracts. International Conference on African Development Issues (CUICADI). Materials Teclmology Track, 154-157.
Bah, S., Diallo, D., Dembélé, S. and Paulsen, B.S. (2006). Ethnopharmacological survey of plants used for the treatment of shistosomiasis in nioro district, mali. Journal of Ethnopharmacology, 105: 387-399.
Colthrup, N.B., Daly, L.H. and Wiberley, S.E. (1990). Introduction to infrared and Raman spectroscopy. Academic Press, Califonia, Pp 1–73.
David, M.S., Jeff, S.C, Nigel B, David, W.J. and Glenda, C.G. (2012). Oxidative stress, antioxidant therapies and chronic kidney disease. Nephrology, 17: 311-321.
David, W.I, William, E.M. and Jeff, M.S. (2014). Urea and ammonia metabolism and the control renal nitrogen excretion. Clinical Journal of the American Society of Nephrology, 10 (8): 31-43.
Ihenetu, S., Ikpa, C.B.C, Njoku, V., Kalu, G, Isiuku, O.B. and Samuel, N. (2020). GC-MS Analysis of Phytochemical Composition, Antibacterial and Antioxidant Properties of Ethanolic Extract of Detarium microcarpum (OFOR). Medicinal and Analytical Chemistry International Journal, 4(1): 000158.
Lapresta-Fernanándéz, A., Fernandéz, A. and Blasco, J. (2012). Nanoecotoxicity effects of engineered silver and gold nanoparticles in aquatic organisms. Trends in Analytical Chemistry, 9:40-59.
Mulvaney, P. (1996). Surface Plasmon spectroscopy of nanosized metal particles. Langmuir, 12(3):788–800.
Narayanan, B. and Sakthive, I.N. (2008). Coriander leaf mediated biosynthesis of gold nanoparticles. Material Letter, 62: 4588–4590.
Owoyele, B.V, Akozoriand, F.A. and Soladoye, A.O. (2006). Anti-ulcer Effect of Leaf Extracts of Nelsonia canescens in Rats. RPMP –Chronic and Common Diseases II, 18:345-349.
Owoyele, B.V, Oloriegbe, Y.Y, Balogun, E.A. and Soladoye, A.O. (2006). Analgesic and anti-inflammatory properties of Nelsonia canescens leaf extract. Journal Ethnopharmacology, 99:153-156.
Shahin, S. A., Naresh, K., Abhinav, L., Angad, S., Hallihosur, S., Abhishek, S. and Utpal, B. (2008). Indian medicinal herbs as sources of antioxidants. Food Research International, 41: 1-15.
Singh, H.P, Gujral, S.S, Sharma, S.K. and Sharma, R.K. (2015). Tannic acid: A natural source to tailor nano crystalline silver particles of different morphologies as antibacterial agent. Advanced Material Letters. 6(12): 1043-1049.
Szymanski, H.A. and Erickson, R.A. (1970). Infrared Absorption Bands. Plenum Press, New York, (2):1-754.
Tsegay, A., Mistre, W., Abebe, E., Aster, T, Gebre, T., Feven, T. and Gebreslassie G. (2020). Assessment of liver and renal function tests among gasoline exposed gas station workers in Mekelle city, Tigray region, NorthernEthiopia. PLOS ONE, 15(10): e0239716.
Vagvala, S.H. and O'Connor, S.D. (2018). Imaging of abnormal liver function tests. Clinical Liver Disease (Hoboken), 11(5):128-134.
Varshneya, R., Bhadauria, S. and Gaur, M.S. (2010). Biogenic synthesis of silver nanocubes and nanorods using sundried Stevia rebaudiana leaves. Advanced Material Letters, 1(3):232-237.
Yakubu, E.O, David, C.B, Moses, Z, Isaac, J.U. and Michael, S.A. (2022)a. Kidney function status of Wistar rat treated with ethanolic extract of Phyllanthus amarus leaves owing to diethylnitrosamine intoxication. Asian Journal Natural Products Biochemistry, 20(1): 6-9.
Yakubu, O.E, Otitoju, O, Isaac, J.U, Atanu, F.O, Bando, C.D. and Auwal, H. (2022)b. Hepatoprotective Effects of Phyllanthus Amarus Ethanolic Extract On CCL4-Induced Hepatotoxicity in Wistar Rats. International Journal of Scientific Research and Innovative Studies, 1(2):50-55.
Yan, J. L, Jie-ping, Y, Zhao, H. S. and Wang, L.G. (2004). Extract reverses CCL4 – induced liver fibrosis in rats. World Journal of Gastroenterology, 10:1037 -1042.