Hypoglycemic Potentials of Newbouldia laevis Stem Bark Extract in Alloxan-Induced Diabetic Rats
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10.58578/ajbmbr.v1i1.3351
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Abstract
This study was carried out to investigate the antidiabetic of the methanol extract of Newbouldia stem bark and to determine its safety and toxicity. Alloxan (150 mg/kg) was administered to albino rats via the oral route. The diabetic rats were then placed in 6 groups, following stabilization of hyperglycemia. The first group was non-diabetic, the second and third group were the diabetic control. The next three groups received, each day, 100, 200 and 400 mg/kg body weight of the methanol extract Newbouldia laevis and the second group received a reference standard, metformin (200mg/kg). Treatment was via the oral route for 14 days and fasting blood sugar level was monitored over this period. Acute toxicity (oral and intraperitoneal) studies on the extract was carried out. Blood glucose levels from day 1 to 14 days of treatment increased significantly (p <0.05) in all the treatment groups, except group 3 (positive control) which showed no significant difference compared to the normal control group. This study supports the use of Newbouldia laevis in traditional medicine as well as highlights the need to further explore the potentials of the plant extract as an antidiabetic.
Keywords:
Hypoglycemic; Newbouldia laevis; Stem Bark; Extract; Alloxan-Induced; Diabetic Rats
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Umaru, I. J., Chisom, G. F., Umaru, K. I., Tansaba, A. A., Nanmar, C., & Ugoeze, E. S. (2024). Hypoglycemic Potentials of Newbouldia laevis Stem Bark Extract in Alloxan-Induced Diabetic Rats. African Journal of Biochemistry and Molecular Biology Research, 1(1), 23-34. https://doi.org/10.58578/ajbmbr.v1i1.3351
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References
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Chen, Y., Yang, D. and Cheng, B. (2020). Clinical characteristics and outcomes of patients with diabetes and COVID-19 in association with glucose-lowering medication. Diabetes Care;43:1399–407.
Ezuruike, U. and Prieto, J.M. (2014). The use of plants in the traditional management of diabetes in Nigeria:pharmacological and toxicological considerations. J Ethnopharmacol;155(2):857–924.
Abdel-lateif, K.S., Maghrabi, I.A. and Eldeab, H.A. (2016). Plants source with therapeutic benefits against diseases. J Bioprocess Biotech;6(293):1–7.
Modi, P. (2007). Diabetes beyond insulin: Review of new drugs for treatment of diabetes mellitus. Curr Drug Discov Technol. 4(1):39-47.
Jayakumar, R. V. (2010). Herbal medicines for Type 2 diabetes: Guest editorial. International Journal of Diabetes for Developing Countries. 30 (3): 111-112.
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Bafor, E. and Sanni, U. (2009). Uterine contractile effects of the aqueous and ethanol leaf extracts of Newbouldia laevis (Bignoniaceae) in vitro. Indian Journal of Pharmaceutical Science, 71(2): 124–127.
Hassan, S. W., Salawu, K., Ladan, M. J., Hassan, L G., Umar, R. A. and Fatihu, M. Y. (2010). Hepatoprotective, Antioxidant and phytochemical properties of leaf extracts of Newbouldia laevis. International J. of Pharm. Tech. Research 2(1); 573-584.
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Andrede-Cetto, A. and Heinrich M. (2005). Mexican plants with hypoglycaemic effect used in the treatment of diabetes. J Ethnopharmacol. 99(3):325- 48.
Bosha, J.A., Anaga, A.O. and Asuzu, I.U. (2015). Bioassay-guided isolation and structural elucidation of antidiabetic principle of methanol leaf extract of Newbouldia laevis (P Beauv). J Pharm Pharmacol. 3:515-26.
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Bodmer, M., Meier, C., Krahenbuhl, S., Jick, S.S. and Meier, C.R. (2008). Metformin, sulfonylureas, or other antidiabetes drugs and the risk of lactic acidosis or hypoglycemia: A nested case-control analysis. Diabetes Care. 31:2086–2091.
Catalan, V.S., Couture, J.A. and LeLorier, J. (2001). Predictors of persistence of use of the novel antidiabetic agent acarbose. Arch. Intern. Med. 161:1106–1112.
Okoli E. C, I. J. Umaru, O. Olawale. (2022). Assessment of phytochemical compositions, antibacterial effects and DPPH scavenging activities of ethanolic root extracts of Pterocarpus erinaceus. Asian Journal of Natural Product Biochemistry, 20(2). DOI: 10.13057/biofar/f200203
Anaduaka, E.G., Ogugua, V.N., Agu, C.V. and Okonkwo, C.C. (2014). Ethanol extracts of Newbouldia laevis stem and leaves modulate serum liver marker enzymes and antioxidant enzymes activities in diabetic rats. Afr J Biotechnol. 13(22): 2265-2272.
Imo, C., Uhegbu, F. O., and Ifeanacho, N. G. (2015). Effect of Exposure to Inhalation of Selected Petroleum Products on Liver Function of Male Albino Rats: A Comparative Study. IOSR Journal of Environmental Science, Toxicology and Food Technology, 19, 2319-2399.
Johnson, R. C., Leopold, J. A., and Loscalzo, J. (2006). Vascular Calcification: PathobiologicalMechanisms and Clinical Implications. Circulation Research, 99(10), 1044-1059.
Schoppet, M., and Shanahan, C. M. (2008). Role for Alkaline Phosphatase as an Inducer of Vascular Calcification in Renal Failure. Kidney International, 73(9), 989-991.
Xiao, Q., Murphy, R. A., Houston, D. K., Harris, T. B., Chow, W. H., and Park, Y. (2013). Dietary and Supplemental Calcium Intake and Cardiovascular Disease Mortality: The National Institutes of Health–AARP diet and health study. JAMA internal medicine, 173(8), 639-646.
Wannamethee, S. G., Sattar, N., Papcosta, O., Lennon, L., and Whincup, P. H. (2013). AlkalinePhosphatase, Serum Phosphate, and Incident Cardiovascular Disease and Total Mortality in Older Men. Arteriosclerosis, Thrombosis, and Vascular Biology, 33(5), 1070-1076.
Chang, W., Chen, L. and Hatch, G.M. (2014). Berberine as a therapy for type 2 diabetes and its complications: from mechanism of action to clinical studies. Biochem Cell Bio;1(5):1–8.
Kaur, G. and Meena C. (2012). Amelioration of obesity, glucose intolerance and oxidative stress in high-fat diet and low dose streptozotocin-induced diabetic rats by combination consisting of curcumin with piperine and quercetin. ISRN Pharmacol, pp 1–7.
Chen, Y., Yang, D. and Cheng, B. (2020). Clinical characteristics and outcomes of patients with diabetes and COVID-19 in association with glucose-lowering medication. Diabetes Care;43:1399–407.
Ezuruike, U. and Prieto, J.M. (2014). The use of plants in the traditional management of diabetes in Nigeria:pharmacological and toxicological considerations. J Ethnopharmacol;155(2):857–924.
Abdel-lateif, K.S., Maghrabi, I.A. and Eldeab, H.A. (2016). Plants source with therapeutic benefits against diseases. J Bioprocess Biotech;6(293):1–7.
Modi, P. (2007). Diabetes beyond insulin: Review of new drugs for treatment of diabetes mellitus. Curr Drug Discov Technol. 4(1):39-47.
Jayakumar, R. V. (2010). Herbal medicines for Type 2 diabetes: Guest editorial. International Journal of Diabetes for Developing Countries. 30 (3): 111-112.
Ezeigbo, I. I. (2010). The Antidiabetic Activities of the Methanolic Leaf Extract of Hymenocardi aacida (Tul). Published MSc.dissertation. University of Nigeria, Nsukka. 8 – 22pp.
De-Silva, T. (1997). Medicinal plants for forest conservation and health care: industrial utilization of medicinal plants in developing countries. Global Initiative for Traditional Systems (gifts) of Health. Journal of Food and Agricultural Organization of the United Nations, 11: 34-44.
Lewis, S. M., Bain, B. J. and Bates, I. (2001). Dacie and Lewis: P ractical Haematology. Ninth Edition, London, New York: Elsevier, 9- 40pp.
Riby, J. E. (2006). Herbal medicines and phytochemicals of indegenoues plants in California. Berkely Molecular and Pharmarcology Journal, 69(2): 430-439.
Arbonnier, M. (2004). Trees, shrubs and lianas of west African dry zones. Germany: CIRAD Press Ltd. 243 – 248pp.
Bafor, E. and Sanni, U. (2009). Uterine contractile effects of the aqueous and ethanol leaf extracts of Newbouldia laevis (Bignoniaceae) in vitro. Indian Journal of Pharmaceutical Science, 71(2): 124–127.
Hassan, S. W., Salawu, K., Ladan, M. J., Hassan, L G., Umar, R. A. and Fatihu, M. Y. (2010). Hepatoprotective, Antioxidant and phytochemical properties of leaf extracts of Newbouldia laevis. International J. of Pharm. Tech. Research 2(1); 573-584.
Tanko, Y., Okasha, M. A., Sale, M. I. A., Mohammed, A., Yerima, M., Yaro, A. H. and Isa, A. I. (2008). Antidiabetic effect of ethanolic flower extracts of Newbouldia laevis (Bignoniaceae) on blood glucose levels of streptozocin-induced diabetic wister rats. Research Journal of Medical sciences 2 (2); 62-65.
Andrede-Cetto, A. and Heinrich M. (2005). Mexican plants with hypoglycaemic effect used in the treatment of diabetes. J Ethnopharmacol. 99(3):325- 48.
Bosha, J.A., Anaga, A.O. and Asuzu, I.U. (2015). Bioassay-guided isolation and structural elucidation of antidiabetic principle of methanol leaf extract of Newbouldia laevis (P Beauv). J Pharm Pharmacol. 3:515-26.
Kolawole, O.T., Akanji, M.A. and Akiibinu, M.O. (2013). Toxicological assessment of ethanolic extract of the leaves of Newbouldia laevis (P. Beauv). Am J Med Med Sci. 3(4):74-80.
Mbagwu, I.S., Akah, P.A., Ajaghaku, A.A., Ugwu, O.C. and lotanna, Ajaghaku, D. (2021). Inhibition of oxidative stress and gastric emptying as additional mechanisms of antidiabetic activity of Newbouldia laevis. Phytomed Plus. 2021; 1(2): 100023.
Osigwe, C.C., Akah, P.A., Nworu, C.S. and Okoye, F.B.C. (2017b). Apigenin: A methanol fraction component of Newbouldia laevis leaf, as a potential antidiabetic agent. J Phytopharmacol, 6(1):38-44.
McCrimmon, R.J. and Sherwin, R.S. (2010). Hypoglycemia in type 1 diabetes. Diabetes. 59:2333–2339.
Paulweber, B., Valensi, P., Lindstrom, J., Lalic, N.M., Greaves, C.J., McKee, M. and Yilmaz, T. A. (2010). European evidence-based guideline for the prevention of type 2 diabetes. Horm. Metab. Res. 42:S3–S36.
Bodmer, M., Meier, C., Krahenbuhl, S., Jick, S.S. and Meier, C.R. (2008). Metformin, sulfonylureas, or other antidiabetes drugs and the risk of lactic acidosis or hypoglycemia: A nested case-control analysis. Diabetes Care. 31:2086–2091.
Catalan, V.S., Couture, J.A. and LeLorier, J. (2001). Predictors of persistence of use of the novel antidiabetic agent acarbose. Arch. Intern. Med. 161:1106–1112.
Okoli E. C, I. J. Umaru, O. Olawale. (2022). Assessment of phytochemical compositions, antibacterial effects and DPPH scavenging activities of ethanolic root extracts of Pterocarpus erinaceus. Asian Journal of Natural Product Biochemistry, 20(2). DOI: 10.13057/biofar/f200203
Anaduaka, E.G., Ogugua, V.N., Agu, C.V. and Okonkwo, C.C. (2014). Ethanol extracts of Newbouldia laevis stem and leaves modulate serum liver marker enzymes and antioxidant enzymes activities in diabetic rats. Afr J Biotechnol. 13(22): 2265-2272.
Imo, C., Uhegbu, F. O., and Ifeanacho, N. G. (2015). Effect of Exposure to Inhalation of Selected Petroleum Products on Liver Function of Male Albino Rats: A Comparative Study. IOSR Journal of Environmental Science, Toxicology and Food Technology, 19, 2319-2399.
Johnson, R. C., Leopold, J. A., and Loscalzo, J. (2006). Vascular Calcification: PathobiologicalMechanisms and Clinical Implications. Circulation Research, 99(10), 1044-1059.
Schoppet, M., and Shanahan, C. M. (2008). Role for Alkaline Phosphatase as an Inducer of Vascular Calcification in Renal Failure. Kidney International, 73(9), 989-991.
Xiao, Q., Murphy, R. A., Houston, D. K., Harris, T. B., Chow, W. H., and Park, Y. (2013). Dietary and Supplemental Calcium Intake and Cardiovascular Disease Mortality: The National Institutes of Health–AARP diet and health study. JAMA internal medicine, 173(8), 639-646.
Wannamethee, S. G., Sattar, N., Papcosta, O., Lennon, L., and Whincup, P. H. (2013). AlkalinePhosphatase, Serum Phosphate, and Incident Cardiovascular Disease and Total Mortality in Older Men. Arteriosclerosis, Thrombosis, and Vascular Biology, 33(5), 1070-1076.
